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Fluorescein-5-maleimide (ultra pure)

Thiol dye
 
ENZ-52502 25 mg 279.00 USD
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Replaces Prod. #: ENZ-52505

Fluorescein-5-maleimide enables protein labeling via thiol modifications in most proteins at cysteine residues that either are intrinsically present or resulted from reduction of cystine residues. Unlike iodoacetamides, maleimides do not react with histidine and methionine under physiological conditions. The probe is suitable for assessing conformation- dependent accessibility of cysteine residues in proteins. Wavelength Maxima: Excitation 493nm, Emission 515nm

Product Specification

Formula:C24H13NO7
 
MW:427.4
 
CAS:75350-46-8
 
Purity:≥95% (HPLC)
 
Appearance:Red powder.
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Conformation-dependent accessibility of Cys-136 and Cys-155 of the mitochondrial rat carnitine/acylcarnitine carrier to membrane-impermeable SH reagents: N. Giangregorio, et al.; Biochim. Biophys. Acta 1767, 1331 (2007), Abstract;
Drug-induced conformational changes in multidrug efflux transporter AcrB from Haemophilus influenzae: V. Dastidar, et al.; J. Bacteriol. 189, 5550 (2007), Abstract;

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