Monobromobimane (ultra pure)

Thiol dye



ENZ-52501	25 mg	84.00 USD

Monobromobimane (mBBr) is a thiol-reactive fluorescent probe used for the determination of the redox status of low molecular weight and protein thiols in biological systems. Monobromobimane-based in situ derivatization results in maximal recovery of both free, reduced low molecular weight and monobromobimane-accessible protein thiols. The quantitation of the corresponding adducts of protein thiols is achieved by fluorescence spectroscopy, following protein precipitation. The reagent is particularly useful for quantifying glutathione.

Alternative Name:	mBBr, 3-(Bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo(1,2-α) pyrazole-1,7-dione

Formula:	C₁₀H₁₁BrN₂O₂

MW:	271.1

Purity:	≥95% (HPLC)

CAS:	71418-44-5

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

Simultaneous and trace determination of reduced and oxidized glutathione in minute plasma samples using dual mode fluorescence detection and column switching high performance liquid chromatography: A.K. Sakhi, et al.; J. Chromatogr. A 1142, 178 (2007), Abstract;

An improved monobromobimane assay for glutathione utilizing tris- (2-carboxyethyl)phosphine as the reductant: M.T. Anderson, et al.; Anal. Biochem. 272, 107 (1999), Abstract;