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Monobromobimane (ultra pure)

Thiol dye
 
ENZ-52501 25 mg 98.00 USD
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Monobromobimane (mBBr) is a thiol-reactive fluorescent probe used for the determination of the redox status of low molecular weight and protein thiols in biological systems. Monobromobimane-based in situ derivatization results in maximal recovery of both free, reduced low molecular weight and monobromobimane-accessible protein thiols. The quantitation of the corresponding adducts of protein thiols is achieved by fluorescence spectroscopy, following protein precipitation. The reagent is particularly useful for quantifying glutathione. Wavelength Maxima: Excitation 395nm, Emission 490nm

Product Specification

Alternative Name:mBBr, 3-(Bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo(1,2-α) pyrazole-1,7-dione
 
Formula:C10H11BrN2O2
 
MW:271.1
 
CAS:71418-44-5
 
Purity:≥95% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Simultaneous and trace determination of reduced and oxidized glutathione in minute plasma samples using dual mode fluorescence detection and column switching high performance liquid chromatography: A.K. Sakhi, et al.; J. Chromatogr. A 1142, 178 (2007), Abstract;
An improved monobromobimane assay for glutathione utilizing tris- (2-carboxyethyl)phosphine as the reductant: M.T. Anderson, et al.; Anal. Biochem. 272, 107 (1999), Abstract;

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