Dansyl chloride demonstrates amine-specific fluorescence (557 nm emission), enabling environmentally sensitive detection in biophysical studies. Dansyl chloride reacts with both aliphatic and aromatic primary amines to produce a stable blue-green fluorescent sulfonamide adduct. In general, the probe serves as a good acceptor for fluorescence resonance energy transfer (FRET) when tryptophan is used as the donor. Alternatively, the dansyl moiety can serve as the donor and polydiacetylene as the acceptor in liposome studies.
The probe is particularly useful for preparing fluorescent drug or ligand analogs that bind to hydrophobic sites in proteins, membranes or other biological receptors. Wavelength Maxima: Excitation 372nm
Product Specification
| Alternative Name: | 5-Dimethylamino-1-naphthalenesulfonyl chloride |
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| Formula: | C12H12ClNO2S |
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| MW: | 269.7 |
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| Purity: | ≥95% (HPLC) |
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| CAS: | 605-65-2 |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Hazard: | CORROSIVE. IRRITANT. |
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| Handling: | Keep cool and dry. Protect from light. |
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| Background / Technical Information: | This product is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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Product Literature References
A ’turn-on’ FRET peptide sensor based on the mercury binding protein MerP: B.R. White, et al.; Analyst
133, 65 (2008),
Abstract;
Modulating fluorescence resonance energy transfer in conjugated liposomes: X. Li, et al.; Langmuir
22, 8615 (2006),
Abstract;