AMCA-NHS (ultra pure)

Amine reactive dye



ENZ-52454	10 mg	81.00 USD

AMCA is a blue fluorescent dye whose reactive derivatives are commonly used as contrasting probes for double and triple labeling in immunofluorescence microscopy, microarrays and in situ hybridization. Desirable properties of AMCA include a relatively large Stoke’s shift and resistance to photobleaching. AMCA-NHS is reactive towards primary amine groups on proteins, peptides and other biomolecules. Wavelength Maxima: Excitation 353nm, Emission 442nm

Product Specification

Alternative Name:	Aminomethylcoumarin acetate succinimidyl ester

MW:	330.3

Purity:	≥95% (HPLC)

CAS:	113721-87-2

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Hazard:	HARMFUL.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

Imaging of fluorescent neurons labelled with fluoro-gold and fluorescent axon terminals labelled with AMCA (7-amino-4-methylcoumarine-3-acetic acid) conjugated antiserum using a UV-laser confocal scanning microscope: B. Ulfhake, et al.; J. Neurosci. Methods 40, 39 (1991), Abstract;

A spectrophotometric method for determination of fluorophore-to-protein ratios in conjugates of the blue fluorophore 7-amino-4-methylcoumarin-3-acetic acid (AMCA): M.W. Wessendorf, et al.; J. Histochem. Cytochem. 38, 87 (1990), Abstract;