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DAPI (ultra pure)

DNA dye
 
ENZ-52404 100 mg 562.00 USD
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DAPI is a fluorescent dye that binds nucleic acids and demonstrates a high affinity for DNA. It is used extensively in fluorescence microscopy, flow cytometry and microplate assays. DAPI is cell permeable and is readily used for detecting both live, dead and fixed cells. DAPI emits in the blue spectral range and is ideal for multiplexed assays inclusive of green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Aside from labeling cell nuclei, DAPI is also used for the detection of mycoplasma or viral DNA in cell cultures. Wavelength Maxima: Excitation 358nm, Emission 461nm

Product Specification

Alternative Name:4’,6-Diamidino-2-phenylindole . dihydrochloride
 
Formula:C16H17Cl2N5
 
MW:350.3
 
CAS:28718-90-3
 
Purity:≥95% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
ENZ-52404 structure

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ENZ-52404 structure

Product Literature References

Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry--new prospects for plant research: J. Suda & P. Travnicek; Cytometry A 69, 273 (2006), Abstract;
DAPI fluorescence in nuclei isolated from tumors: A. Krishan & P.D. Dandekar; J. Histochem. Cytochem. 53, 1033 (2005), Abstract;

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