DAPI (ultra pure)

DNA dye



ENZ-52404	100 mg	493.00 USD

DAPI is a fluorescent dye that binds nucleic acids and demonstrates a high affinity for DNA. It is used extensively in fluorescence microscopy, flow cytometry and microplate assays. DAPI is cell permeable and is readily used for detecting both live, dead and fixed cells. DAPI emits in the blue spectral range and is ideal for multiplexed assays inclusive of green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Aside from labeling cell nuclei, DAPI is also used for the detection of mycoplasma or viral DNA in cell cultures.

Alternative Name:	4’,6-Diamidino-2-phenylindole . dihydrochloride

Formula:	C₁₆H₁₇Cl₂N₅

MW:	350.3

Purity:	≥95% (HPLC)

CAS:	28718-90-3

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Hazard:	IRRITANT.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry--new prospects for plant research: J. Suda & P. Travnicek; Cytometry A 69, 273 (2006), Abstract;

DAPI fluorescence in nuclei isolated from tumors: A. Krishan & P.D. Dandekar; J. Histochem. Cytochem. 53, 1033 (2005), Abstract;