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Hoechst 33342 (ultra pure)

Cell permeable DNA dye
 
ENZ-52401 100 mg 128.00 USD
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Replaces Prod. #: BML-GR303

Hoechst 33342 is fluorescent probe useful for detecting DNA by fluorescence microscopy and flow cytometry. Hoechst 33342 may be used on live or fixed cells and is also applicable for cell cycle analysis and monitoring DNA condensation by flow cytometry. An additional ethyl group renders Hoechst 33342 more lipophilic, and thus more readily cell permeable than Hoechst 33258 . Exclusion of Hoechst 33342 dye is a characteristic common to stem cells, as well as chemotherapy-resistant cancer cells. Wavelength Maxima: Excitation 350nm, Emission 461nm

Product Specification

Alternative Name:2’-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole
 
Formula:C27H37Cl3N6O4
 
MW:561.9
 
CAS:23491-52-3
 
Purity:≥95% (HPLC)
 
Appearance:Green solid.
 
Solubility:Soluble in water (50mg/ml) or DMSO (10mg/ml).
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

FOXO3 is essential for CD44 expression in pancreatic cancer cells: M. Kumazoe, et al.; Oncogene 36, 2643 (2017), Abstract;
Development of alphaviral vectors for macrophage activation in cancer immunotherapy: B. Kurena; (2016), (master's thesis), Application(s): Cell immunostaining, Full Text
Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells: J.H. Kim, et al.; Int. J. Nanomedicine 10, 5513 (2015), Application(s): Cell culture of human cells, Abstract; Full Text
Effects of mechanical stretching on the morphology and cytoskeleton of vaginal fibroblasts from women with pelvic organ prolapse: S. Wang, et al.; Int. J. Mol. Sci. 16, 9406 (2015), Application(s): Cell Culture, Microscopy, Abstract; Full Text
Utilization of fluorescent probes for the quantification and identification of subcellular proteomes and biological processes regulated by lipid peroxidation products: T.D. Cummins, et al.; Free Radic. Biol. Med. 59, 56 (2013), Application(s): Cell Culture, Abstract; Full Text
Hormone-induced 14-3-3γ adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells: Y. Aghazadeh, et al.; J. Biol. Chem. 287, 15380 (2012), Application(s): Nuclear staining of Adult mouse testis sections, Abstract; Full Text
Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid, et al.; Nat. Protoc. 2, 187 (2007), Abstract;
High-throughput microtiter assay for Hoechst 33342 dye uptake: G.M. Seigel & L.M. Campbell; Cytotechnology 45, 155 (2004), Abstract;

Related Products

Hoechst 33258 (ultra pure) 

DNA dye
23491-45-4, ≥95% (HPLC) | Print as PDF
 
ENZ-52402 100 mg 128.00 USD
Do you need bulk/larger quantities?
 

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