Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1,TMRE is widely used for measuring mitochondrial membrane potential, in addition to selectively staining mitochondria. Real-time imaging of mitochondrial membrane potential in individual cardiomyocytes within perfused rat hearts has been demonstrated with this dye, using 2-photon laser-scanning microscopy. Wavelength Maxima: Excitation 549nm, Emission 574nm
Product Details
| Alternative Name: | Tetramethylrhodamine ethyl ester perchlorate, 3,6-bis(Dimethylamino)-9-[2-(ethoxycarbonyl)phenyl]-xanthylium perchlorate |
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| Formula: | C26H27ClN2O7 |
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| MW: | 515.0 |
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| CAS: | 115532-52-0 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
PUMILIO hyperactivity drives premature aging of Norad-deficient mice: F. Kopp, et al.; Elife
8, e42650 (2019),
Application(s): Flow cytometry using immortalized MEFs,
Abstract;
Full Text
Autophagy maintains the metabolism and function of young and old (hematopoietic) stem cells: T.T. Ho, et al.; Nature
543, 205 (2017),
Abstract;
Full Text
The role of c-FLIP splice variants in urothelial tumours: F. Ewald, et al.; Cell Death Dis.
2, e245 (2011),
Abstract;
Full Text
The mitochondrial membrane potential and Ca2+ oscillations in smooth muscle: S. Chalmers & J.G. McCarron; J. Cell Sci.
121, 75 (2008),
Abstract;
Real-time 2-photon imaging of mitochondrial function in perfused rat hearts subjected to ischemia/reperfusion: M. Matsumoto-Ida, et al.; Circulation
114, 1497 (2006),
Abstract;
General Literature References
Quantitative measurement of mitochondrial membrane potential in cultured cells: calcium-induced de- and hyperpolarization of neuronal mitochondria: A. A. Gerencser, et. al.; J. Physiol.
590, 2845 (2012),
Abstract;
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