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Rhodamine 123 (ultra pure)

Mitochondria dye
 
ENZ-52307 25 mg 98.00 USD
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Replaces Prod. #: ALX-610-018

Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye that is readily sequestered by active mitochondria without cytotoxic effects. Rhodamine 123 can be used in multi-parametric analysis, without fluorescence interference, in combination with common protein labeling dyes such as cyanine-5 and AMCA. With respect to apoptosis, the presence of mitochondrial membrane potential can be probed with rhodamine 123 while the structure and integrity of mitochondria can be assessed using 10-N-nonyl-acridine orange. Wavelength Maxima: Excitation 507nm, Emission 529nm

Product Specification

Alternative Name:3,6-Diamino-9-(2-(methoxycarbonyl)phenyl xanthylium chloride
 
Formula:C21H17ClN2O3
 
MW:380.8
 
CAS:62669-70-9
 
Purity:≥95% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Detection of de- and hyperpolarization of mitochondria of cultured astrocytes and neurons by the cationic fluorescent dye rhodamine 123: S. Kahlert, et al.; J. Neurosci. Methods 171, 87 (2008), Abstract;
Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange: C. Ferlini & G. Scambia; Nat. Protoc. 2, 3111 (2007), Abstract;

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