10-N-Nonyl acridine orange (NAO) is an acridine orange derivative and is generally used as a fluorescent marker of the inner mitochondrial membrane in whole cells. It is believed to bind to negatively charged phospholipids. NAO accumulation in the cell seems to be related to specific interactions with mitochondrial membrane proteins and/or lipids, such as cardiolipin, and is largely independent of mitochondrial membrane potential. With respect to apoptosis, the presence of mitochondrial membrane potential can be probed with Rhodamine123 while the structure and integrity of mitochondria can be assessed using 10-N-nonyl-acridine orange. Wavelength Maxima: Excitation 495nm, Emission 519nm
Product Details
| Alternative Name: | Nonyl Acridine Orange, 3,6-bis(Dimethylamino)-10-nonyl-acridinium bromide |
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| MW: | 472.5 |
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| CAS: | 75168-11-5 |
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| Purity: | ≥95% (HPLC). |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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