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JC-10 (ultra pure)

Mitochondria dye
 
ENZ-52305 5 mg 218.00 USD
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JC-10 is a derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. However, upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (525 nm). The principal advantages of JC-10 relative to JC-1 include improved solubility in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. JC-10 allows for both qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio, of mitochondrial membrane potential changes. Wavelength Maxima: Excitation 510nm, Emission 525nm

Product Specification

Alternative Name:Enhanced JC-1
 
MW:~600
 
Purity:≥95% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 

Product Literature References

MKK3 influences mitophagy and is involved in cigarette smoke-induced inflammation: P. Mannam, et al.; Free Radic. Biol. Med. 101, 102 (2016), Application(s): Detecting Mitochondrial Membrane Potential (MMP) in BMDMs, Abstract;
PINK1 and Parkin cooperatively protect neurons against constitutively active TRP channel-induced retinal degeneration in Drosophila: Z. Huang, et al.; Cell Death Dis. 7, e2179 (2016), Application(s): Mitochondrial membrane potential was measured by staining with 25 μM JC-10 reagent, Abstract; Full Text
PINK1-dependent phosphorylation of PINK1 and Parkin is essential for mitochondrial quality control: N. Zhuang, et al.; Cell Death Dis. 7, e2501 (2016), Abstract; Full Text
Induced peroxidase and cytoprotective enzyme expressions support adaptation of HUVECs to sustain subsequent H₂O₂ exposure: H. Patel, et al.; Microvasc. Res. 2862, 30025 (2015), Application(s): Measument of HUVECs mitochondrial membrane polarization levels, Abstract;

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