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Dihydrorhodamine 123 (ultra pure)

Mitochondria dye
 
ENZ-52302 10 mg 160.00 USD
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Replaces Prod. #: ALX-610-021

Dihydrorhodamine 123 is a widely utilized probe for detection of intracellular reactive oxygen species (ROS) such as peroxide, hypochlorous acid and peroxynitrite. It is readily oxidized into rhodamine 123, which exhibits a spectral profile similar to that of FITC. In combination with other fluorescent probes (such as surface receptor-targeted fluorescent antibodies, the cell viability probe propidium iodide, or fluorescent calcium indicators) dihydrorhodamine 123 can be used for multiparametric cell measurements. Wavelength Maxima: Excitation 507nm, Emission 529nm

Product Specification

Alternative Name:2-(3,6-Diamino-9H-xanthene-9-yl)-benzoic acid methyl ester
 
MW:346.4
 
CAS:109244-58-8
 
Purity:≥95% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Oxidative burst assessment and neutrophil-platelet complexes in unlysed whole blood: A. Avendano, et al.; J. Immunol. Methods 339, 124 (2008), Abstract;
Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD: L. Mauch, et al.; Clin. Chem. 53, 890 (2007), Abstract;

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