DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environmentsensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The carbocyanine dye DiIC1(5) has been used to measure mitochondrial membrane potential in apoptotic cells with a loss in membrane potential reflected in a loss in fluorescence signal in the infrared channel. Wavelength Maxima: Excitation 638nm, Emission 658nm
Product Details
| Alternative Name: | 1,1’,3,3,3’,3’-Hexamethylindodicarbocyanine iodide |
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| MW: | 510.5 |
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| CAS: | 36536-22-8 |
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| Purity: | ≥98% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Construction and validation of a novel algorithm based on oncosis‑related lncRNAs comprising the immune landscape and prediction of colorectal cancer prognosis: H. Xiang, et al.; Oncol. Lett.
25, 63 (2023),
Abstract;
Flow cytometric analysis of isolated liver mitochondria to detect changes relevant to cell death: G. Mattiasson; Cytometry A
60, 145 (2004),
Abstract;
Flow cytometric analysis of mitochondria from CA1 and CA3 regions of rat hippocampus reveals differences in permeability transition pore activation: G. Mattiasson, et al.; J. Neurochem.
87, 532 (2003),
Abstract;
