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Di-8-ANEPPS

Membrane Potential Detector
 
ENZ-52204 5 mg 313.00 USD
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Di-8-ANEPPS is a member of the ANEP class of membrane potential dyes. These dyes are weakly fluorescent in aqueous media, and become strongly fluorescent upon binding to lipophilic environments such as membranes. A change in the surrounding electronic field demonstrates a membrane potential-dependent shift in excitation spectra. Di-8-ANEPPS is less susceptible for cellular internalization than other ANEP dyes probably due to a sulfonate group. The response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains. Wavelength Maxima: Excitation 498nm, Emission 713nm

Product Specification

Alternative Name:4-[2-[6-(Dioctylamino)-2-naphthalenyl]ethenyl]-1-(3-sulfopropyl)-pyridinium, inner salt
 
Formula:C36H52N2O3S
 
MW:592.9
 
CAS:157134-53-7
 
Purity:≥90% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:

This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

 
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Product Literature References

Stimulatory actions of di-8-butyl-amino-naphthyl-ethylene-pyridinium-propyl-sulfonate (di-8-ANEPPS), voltage-sensitive dye, on the BKCa channel in pituitary tumor (GH3) cells: S.N. Wu, et al.; Pflugers Arch. 455, 687 (2008), Abstract;

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