Di-8-ANEPPS

Membrane Potential Detector



ENZ-52204	5 mg	266.00 USD

Di-8-ANEPPS is a member of the ANEP class of membrane potential dyes. These dyes are weakly fluorescent in aqueous media, and become strongly fluorescent upon binding to lipophilic environments such as membranes. A change in the surrounding electronic field demonstrates a membrane potential-dependent shift in excitation spectra. Di-8-ANEPPS is less susceptible for cellular internalization than other ANEP dyes probably due to a sulfonate group. The response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains.

Alternative Name:	4-[2-[6-(Dioctylamino)-2-naphthalenyl]ethenyl]-1-(3-sulfopropyl)-pyridinium, inner salt

Formula:	C₃₆H₅₂N₂O₃S

MW:	592.9

Purity:	≥90% (HPLC)

CAS:	157134-53-7

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

Stimulatory actions of di-8-butyl-amino-naphthyl-ethylene-pyridinium-propyl-sulfonate (di-8-ANEPPS), voltage-sensitive dye, on the BKCa channel in pituitary tumor (GH3) cells: S.N. Wu, et al.; Pflugers Arch. 455, 687 (2008), Abstract;