Di-8-ANEPPS is a member of the ANEP class of membrane potential dyes. These dyes are weakly fluorescent in aqueous media, and become strongly fluorescent upon binding to lipophilic environments such as membranes. A change in the surrounding electronic field demonstrates a membrane potential-dependent shift in excitation spectra. Di-8-ANEPPS is less susceptible for cellular internalization than other ANEP dyes probably due to a sulfonate group. The response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains. Wavelength Maxima: Excitation 498nm, Emission 713nm
Product Details
| Alternative Name: | 4-[2-[6-(Dioctylamino)-2-naphthalenyl]ethenyl]-1-(3-sulfopropyl)-pyridinium, inner salt |
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| Formula: | C36H52N2O3S |
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| MW: | 592.9 |
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| CAS: | 157134-53-7 |
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| Purity: | ≥90% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Stimulatory actions of di-8-butyl-amino-naphthyl-ethylene-pyridinium-propyl-sulfonate (di-8-ANEPPS), voltage-sensitive dye, on the BKCa channel in pituitary tumor (GH3) cells: S.N. Wu, et al.; Pflugers Arch.
455, 687 (2008),
Abstract;
