5(6)-CDCFDA (ultra pure)

pH indicator



ENZ-52103	100 mg	84.00 USD

5(6)-CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. 5(6)-CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in cells with intact cell membranes. 5(6)-CFCFDA does not stain dead cells. 5(6)-CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells.

Alternative Name:	5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate

Formula:	C₂₅H₁₄Cl₂O₉

MW:	529.3

Purity:	≥98% (HPLC)

CAS:	127770-45-0

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

20-HETE increases superoxide production and activates NAPDH oxidase in pulmonary artery endothelial cells: M. Medhora, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008), Abstract;

The role of oxidative stress in acrolein-induced DNA damage in HepG2 cells: L. Li, et al.; Free Radic. Res. 42, 354 (2008), Abstract;