BCECF AM (2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester) is a well established fluorescent probe that enables ratiometric monitoring of cellular pH. Intracellular pH plays an important role in modulating cellular events, including growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, adhesion and other cellular processes. Once inside, cellular esterases cleave the AM groups yielding a more hydrophilic BCECF trapped inside the cell. Wavelength Maxima: Excitation 505nm, Emission 520nm
Product Details
| Alternative Name: | 2',7'-bis-(2-Carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester |
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| Formula: | C39H36O19 |
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| MW: | 808.7 |
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| CAS: | 117464-70-7 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Off white solid |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Photophysics of the fluorescent pH indicator BCECF: N. Boens, et al.; J. Phys. Chem. A
110, 9334 (2006),
Abstract;
Simultaneous measurement of water volume and pH in single cells using BCECF and fluorescence imaging microscopy: F.J. Alvarez-Leefmans, et al.; Biophys. J.
90, 608 (2006),
Abstract;
