BCECF AM (ultra pure)

pH indicator



ENZ-52102	1 mg	187.00 USD

BCECF AM (2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester) is a well established fluorescent probe that enables ratiometric monitoring of cellular pH. Intracellular pH plays an important role in modulating cellular events, including growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, adhesion and other cellular processes. Once inside, cellular esterases cleave the AM groups yielding a more hydrophilic BCECF trapped inside the cell.

Alternative Name:	2’,7’-bis-(2-Carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester

Formula:	C₃₉H₃₆O₁₉

MW:	808.7

Purity:	≥95% (HPLC)

CAS:	117464-70-7

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Please mouse over

Product Literature References

Photophysics of the fluorescent pH indicator BCECF: N. Boens, et al.; J. Phys. Chem. A 110, 9334 (2006), Abstract;

Simultaneous measurement of water volume and pH in single cells using BCECF and fluorescence imaging microscopy: F.J. Alvarez-Leefmans, et al.; Biophys. J. 90, 608 (2006), Abstract;