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BCECF AM (ultra pure)

pH indicator
 
ENZ-52102 1 mg 223.00 USD
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BCECF AM (2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester) is a well established fluorescent probe that enables ratiometric monitoring of cellular pH. Intracellular pH plays an important role in modulating cellular events, including growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, adhesion and other cellular processes. Once inside, cellular esterases cleave the AM groups yielding a more hydrophilic BCECF trapped inside the cell. Wavelength Maxima: Excitation 505nm, Emission 520nm

Product Specification

Alternative Name:2',7'-bis-(2-Carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester
 
Formula:C39H36O19
 
MW:808.7
 
CAS:117464-70-7
 
Purity:≥95% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
enz-52102
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Product Literature References

Photophysics of the fluorescent pH indicator BCECF: N. Boens, et al.; J. Phys. Chem. A 110, 9334 (2006), Abstract;
Simultaneous measurement of water volume and pH in single cells using BCECF and fluorescence imaging microscopy: F.J. Alvarez-Leefmans, et al.; Biophys. J. 90, 608 (2006), Abstract;

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