BCECF acid (ultra pure)

pH indicator



ENZ-52101	1 mg	187.00 USD

BCECF (2',7'-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein) is a well established cell impermeable, fluorescent probe that enables ratiometric monitoring of cellular pH (pKa 7.0) and can be loaded in cells by microinjection, electroporation or scrape loading. The AM ester form of the dye should be used for passive loading. Intracellular pH plays an important role in modulating cellular events, including growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, adhesion and other cellular processes.

Alternative Name:	2’,7’-bis-(2-Carboxyethyl)-5-(6)-carboxyfluorescein

Formula:	C₂₇H₂₀O₁₁

MW:	520.4

Purity:	≥95% (HPLC)

CAS:	85138-49-4

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

Photophysics of the fluorescent pH indicator BCECF: N. Boens, et al.; J. Phys. Chem. A 110, 9334 (2006), Abstract;

Simultaneous measurement of water volume and pH in single cells using BCECF and fluorescence imaging microscopy: F.J. Alvarez-Leefmans, et al.; Biophys. J. 90, 608 (2006), Abstract;