5-Carboxyfluorescein succinimidyl ester (ultra pure)

Amine reactive dye



ENZ-52053	10 mg	165.00 USD

5-Carboxyfluorescein succinimidyl ester is an aminereactive ester of single isomer 5-Carboxyfluorescein. It has principally been used to develop a variety of green fluorescent reagents and small fluorescent molecules due to its relatively high absorbance characteristics, excellent fluorescence quantum yield, and good water solubility. Carboxyfluorescein succinimidyl ester has recently become a popular probe for labeling cells intracellularly and tracking their mitotic activity by flow cytometry and other fluorescence- based assays through monitoring progressive twofold reductions in fluorescence intensity.

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Product Specification

Alternative Name:	5-FAM-OSu (ultra pure), 2,5-Pyrrolidinedione, 1-[[(3’,6’-dihydroxy-3-oxospiro[isobenzofuran-1(3H), 9’-[9H]xanthen]-5-yl)carbonyl]oxy]-

Formula:	C₂₅H₁₅NO₉

MW:	473.4

Purity:	≥95% (HPLC)

CAS:	92557-80-7

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

Rapidly proliferating CD44hi peripheral T cells undergo apoptosis and delay posttransplantation T-cell reconstitution after allogeneic bone marrow transplantation: S.O. Alpdogan, et al.; Blood 112, 4755 (2008), Abstract;

Suppressive efficacy and proliferative capacity of human regulatory T cells in allogeneic and xenogeneic responses: Y.J. Lin, et al.; Transplantation 86, 1452 (2008), Abstract;

Carboxyfluorescein succinimidyl ester-based proliferative assays for assessment of T cell function in the diagnostic laboratory: D. Fulcher & S. Wong; Immunol. Cell Biol. 77, 559 (1999), Abstract;