GFP-Certified™ FluoForte™ Reagent

Calcium dye



ENZ-52016-5C50	5x50 µg	212.00 USD

ENZ-52016-M001	1 mg	251.00 USD

The GFP-Certified™ FluoForte™ Reagent is the brightest and most sensitive fluorescent calcium indictor relative to other commercially available red fluorescent dyes with maximum excitation at ~530nm and maximum emission at ~555nm. This product is used in a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

Product Specification

MW:	~1’000

Purity:	≥95% (HPLC)

Appearance:	Lyophilized, orange.

Solubility:	Soluble in DMSO.

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Handling:	Avoid freeze/thaw cycles. Protect from light. Protect from moisture.

Background / Technical Information:	The GFP-Certified™ FluoForte™ Reagent is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

General Literature References

Novel fluo-4 analogs for fluorescent calcium measurements: V.V. Martin, et al.; Cell Calcium 36, 509 (2004), Abstract;

Calcium measurements in perfused mouse heart: quantitating fluorescence and absorbance of Rhod-2 by application of photon migration theory: C. Du, et al.; Biophys J. 80, 549 (2001), Full Text

Rhod-2 based measurements of intracellular calcium in the perfused mouse heart: cellular andsubcellular localization and response to positive inotropy: G.A. MacGowan, et al. ; J. Biomed. Opt. 6, 23 (2001), Abstract;

Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41: M. do Ceu Monteiro, et al.; Cytometry 35, 302 (1999), Abstract;

Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3: C. Perez-Terzic, et al.; Cell Calcium 21, 275 (1997), Abstract;

Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets.: R. Greimers, et al. ; Cytometry 23, 205 (1996), Abstract;