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GFP-CERTIFIED® FLUOFORTE® Reagent

Calcium dye
 
ENZ-52016-5C50 5x50 µg 250.00 USD
 
ENZ-52016-M001 1 mg 300.00 USD
Do you need bulk/larger quantities?
 
The GFP-CERTIFIED® FLUOFORTE® Reagent is the brightest and most sensitive fluorescent calcium indictor relative to other commercially available red fluorescent dyes with maximum excitation at ~530nm and maximum emission at ~555nm. This product is used in a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

Product Specification

MW:~1’000
 
Purity:≥95% (HPLC)
 
Appearance:Lyophilized, orange.
 
Solubility:Soluble in DMSO.
 
Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Handling:Protect from moisture. Protect from light. Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:The GFP-CERTIFIED® FLUOFORTE® Reagent is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required. 
 

Product Literature References

Sulfur Dioxide Protects Against Collagen Accumulation in Pulmonary Artery in Association With Downregulation of the Transforming Growth Factor β1/Smad Pathway in Pulmonary Hypertensive Rats: W. Yu, et al.; J. Am. Heart Assoc. 5, e003910 (2016), Application(s): Detection of calcium levels in PAFs and PASMCs, Abstract; Full Text
GABAergic signaling in the pulmonary neuroepithelial body microenvironment: functional imaging in GAD67-GFP mice: K. Schnorbusch, et al.; Histochem. Cell. Biol. 140, 549 (2013), Abstract;
FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ: S. Kumar, et al.; Chemphyschem 12, 609 (2011), Application(s): Calcium levels in HEK293 cells, Abstract; Full Text

General Literature References

Novel fluo-4 analogs for fluorescent calcium measurements: V.V. Martin, et al.; Cell Calcium 36, 509 (2004), Abstract;
Calcium measurements in perfused mouse heart: quantitating fluorescence and absorbance of Rhod-2 by application of photon migration theory: C. Du, et al.; Biophys J. 80, 549 (2001), Full Text
Rhod-2 based measurements of intracellular calcium in the perfused mouse heart: cellular andsubcellular localization and response to positive inotropy: G.A. MacGowan, et al. ; J. Biomed. Opt. 6, 23 (2001), Abstract;
Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41: M. do Ceu Monteiro, et al.; Cytometry 35, 302 (1999), Abstract;
Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3: C. Perez-Terzic, et al.; Cell Calcium 21, 275 (1997), Abstract;
Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets.: R. Greimers, et al. ; Cytometry 23, 205 (1996), Abstract;

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