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FLUOFORTE® Reagent

Calcium dye
 
ENZ-52015 1 mg 294.00 USD
Do you need bulk/larger quantities?
 
Used in a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Fluorescent calcium indicator dye developed to improve cell loading and calcium response while maintaining the convenient Fluo-3, Fluo-4 and Calcium 4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. Relative to the cited dyes, FLUOFORTE® AM dye yields the brightest signal and largest assay window.

Product Specification

Purity:≥95% (HPLC)
 
Appearance:Lyophilized, orange.
 
Solubility:Soluble in DMSO.
 
Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
 
Application Notes:Provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:The FLUOFORTE® Reagent is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 

Product Literature References

Controlling fertilization and cAMP signaling in sperm by optogenetics: V. Jansen, et al.; eLife 4, e05161 (2015), Application(s): Cell Culture, Abstract; Full Text
Dual Optical Recordings for Action Potentials and Calcium Handling in Induced Pluripotent Stem Cell Models of Cardiac Arrhythmias Using Genetically Encoded Fluorescent Indicators: L. Song, et al.; Stem Cells Transl. Med. 4, 468 (2015), Abstract;
Bcl-wav and the mitochondrial calcium uniporter drive gastrula morphogenesis in zebrafish: J. Prudent, et al.; Nat. Commun. 4, 2330 (2013), Application(s): Calcium flux measurement using confocal microscopy, Abstract;
Cysteine (C)-x-C receptor 4 undergoes transportin 1-dependent nuclear localization and remains functional at the nucleus of metastatic prostate cancer cells: A.S. Don-Salu-Hewage, et al. ; PLoS One 8, e57194 (2013), Application(s): Calcium flux measurement using a microplate reader, Abstract; Full Text
Late Sodium Current Inhibition Reverses Electromechanical Dysfunction in Human Hypertrophic Cardiomyopathy: R. Coppini, et al.; Circulation 127, 575 (2013), Application(s): Intracellular Ca2+ mobilization in single myocyte cell using patch-clamp, Abstract; Full Text
Activation of haem-oxidized soluble guanylyl cyclase with BAY 60-2770 in human platelets lead to overstimulation of the cyclic GMP signaling pathway: C.B. Mendes, et al.; PLoS One 7, e47223 (2012), Application(s): Calcium levels in human platelets, Abstract; Full Text
Hedgehog partial agonism drives Warburg-like metabolism in muscle and brown fat: R. Teperino, et al.; Cell 151, 414 (2012), Application(s): Calcium levels in a variety of mouse cells, Abstract;
No evidence for inositol 1,4,5-trisphosphate-dependent Ca2+ release in isolated fibers of adult mouse skeletal muscle: B. Blaauw, et al.; J. Gen. Physiol. 140, 235 (2012), Application(s): Imaging of FluoForte® fluorescence in cultured C2C12 myotubes, Abstract; Full Text

General Literature References

High throughput assay technologies for ion channel drug discovery: W. Zheng, et al.; Assay Drug Dev. Technol. 2, 543 (2004), Abstract;
Novel fluo-4 analogs for fluorescent calcium measurements: V.V. Martin, et al.; Cell Calcium 36, 509 (2004), Abstract;
Signalling specificity in GPCR-dependent Ca2+ signalling: K. Kiselyov, et al.; Cell Signal. 15, 243 (2003), Abstract;
Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41: M. do Ceu Monteiro, et al.; Cytometry 35, 302 (1999), Abstract;
Loading and localization of Fluo-3 and Fluo-3/AM calcium indicators in sinapis alba root tissue: A. Tretyn, et al.; Folia Histochem. Cytobiol. 35, 41 (1997), Abstract;
Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3: C. Perez-Terzic, et al.; Cell Calcium 21, 275 (1997), Abstract;

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