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Quin-2 (ultra pure)

Calcium dye
 
ENZ-52013 5 mg 141.00 USD
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Replaces Prod. #: ALX-620-006

Quin-2 demonstrates a strong affinity for calcium, resulting in a marked shift in ultraviolet absorption and fluorescence spectra versus the unbound state. Quin-2 resembles calcium chelator EGTA in its ability to bind calcium more tightly than magnesium. Quin-2 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Quin-2 has lower absorptivity and quantum yield values than Fura-2, Indo-1, Fluo-3 and Fluo-4, thus requiring higher loading concentrations. Wavelength Maxima: Excitation 353nm, Emission 495nm

Product Specification

Alternative Name:2-[(2-bis-[Carboxymethyl]amino-5-methylphenoxy)-methyl]-6-methoxy-8-bis[carboxymethyl]aminoquinoline
 
Formula:C28H23N3O10 . 4K
 
MW:693.9
 
CAS:73630-23-6
 
Purity:≥95% (HPLC)
 
Appearance:Yellow solid.
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2: J.R. Lakowicz, et al.; Cell Calcium 15, 7 (1994), Abstract;
Fluorescence lifetime imaging of calcium using Quin-2: J.R. Lakowicz, et al.; Cell Calcium 13, 131 (1992), Abstract;

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