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Rhod-2 AM (ultra pure)

Calcium dye
 
ENZ-52010 1 mg 287.00 USD
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Replaces Prod. #: ALX-620-009

Rhod-2 AM has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, making it suitable for use with argon and krypton laser excitation sources. Rhod-2 signal intensity one of the strongest of all the calcium probes. Rhod 2 is especially suitable probe for intracellular calcium monitoring by confocal laser-scanning microscopy and flow cytometry. The dissociation constant of Rhod 2 with calcium is among the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Rhod-2 AM is an acetoxymethyl ester derivative of Rhod-2 that can be easily loaded into cells by passive incubation. Wavelength Maxima: Excitation 549nm, Emission 578nm

Product Specification

Alternative Name:1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N',N'-tetraacetic acid, tetraacetoxy methyl ester . Br
 
MW:1124.0
 
CAS:129787-64-0
 
Purity:≥90% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Calibration of the calcium dissociation constant of Rhod(2)in the perfused mouse heart using manganese quenching: C. Du, et al.; Cell Calcium 29, 217 (2001), Abstract;
Mitochondrial free calcium levels (Rhod-2 fluorescence) and ultrastructural alterations in neuronally differentiated PC12 cells during ceramide-dependent cell death: M.P. Muriel, et al.; J. Comp. Neurol. 426, 297 (2000), Abstract;

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