INDO-1 AM (ultra pure)

Calcium dye



ENZ-52008	1 mg	133.00 USD

Indo-1 acetomethoxy (AM) is a popular UV-excitable calcium indicator, similar to Fura-2. In contrast to Fura-2, Indo-1 has a dual emission peak. The main emission peak in calcium-free solution is 475nm while in the presence of calcium the emission is shifted to 400nm. Indo-1 AM is a membrane permeable probe and can be easily loaded into cells by passive incubation. The probe is widely used in flow cytometry. For this application, it is critical to have an instrument with an argon laser tuned to UV or a heliumcadmium laser available.

Alternative Name:	1-[2-Amino-5-(6-carboxyindol-2-yl)phenoxy]-2-(2\'-amino-5\'-methylphenoxy)ethane-N,N,N\',N\'-tetraacetic acid, pentaacetoxymethylester

Formula:	C₄₇H₅₁N₃O₂₂

MW:	1009.9

Purity:	≥95% (HPLC)

Appearance:	Yellow powder.

CAS:	112926-02-0

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux: S. Bailey & P.J. Macardle; J. Immunol. Methods 311, 220 (2006), Abstract;

Measurement of [Ca2+] in cell suspensions using indo-1: A. Nelemans; Methods Mol. Biol. 312, 47 (2006), Abstract;