INDO-1 AM (Ultra Pure) - Enzo Life Sciences

ENZ-52008

Revised 01-Feb-10

INDO-1 AM (Ultra Pure)
SYNONYMS	1-[2-Amino-5-(6-carboxyindol-2-yl)phenoxy]-2-(2\'-amino-5\'-methylphenoxy)ethane-N,N,N\',N\'-tetraacetic acid, pentaacetoxymethylester
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Chelators / Caged Calcium Chelators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ENZ-52008		1 mg	129.00 USD

Product Specification

MW:	1001.9
CAS NUMBER:	112926-02-0
PURITY:	≥95% (HPLC)
SOLUBILITY:	Soluble in DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least one year after receipt when stored as recommended.
HANDLING:	Keep cool and dry. Protect from light.

Product Description

Indo-1 acetomethoxy (AM) is a popular UV-excitable calcium indicator, similar to Fura-2. In contrast to Fura-2, Indo-1 has a dual emission peak. The main emission peak in calcium-free solution is 475nm while in the presence of calcium the emission is shifted to 400nm. Indo-1 AM is a membrane permeable probe and can be easily loaded into cells by passive incubation. The probe is widely used in flow cytometry. For this application, it is critical to have an instrument with an argon laser tuned to UV or a heliumcadmium laser available. Wavelength Maxima: Excitation 346nm, Emission 475nm

Product Specific Literature References

Measurement of [Ca2+] in cell suspensions using indo-1: A. Nelemans; Methods Mol. Biol. 312, 47 (2006) Abstract
A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux: S. Bailey & P.J. Macardle; J. Immunol. Methods 311, 220 (2006) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION
This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.