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FURA-2 (ultra pure)

Calcium dye
 
ENZ-52007 1 mg 98.00 USD
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Replaces Prod. #: ALX-620-004

Fura-2 is one of the most widely used calcium indicators for homogenous quantitative dual wavelength ratiometric cell measurements. Fura-2 is particularly useful for digital imaging microscopy. It is less susceptible to photobleaching than Indo-1. The probe is excited only by UV light, which results in significantly less interference by visible wavelength excitable fluorescent compounds. One application of the probe is as a useful counter screen tool for GPCR and calcium channels identified using calcium indicators excited at 488nm in primary screens. Fura-2 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Wavelength Maxima: Excitation 363nm, Emission 512nm

Product Specification

Alternative Name:1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid . 5K
 
Formula:C29H22K5N3O14
 
MW:832.0
 
CAS:113694-64-7
 
Purity:≥95% (HPLC)
 
Appearance:Light yellow solid.
 
Solubility:Soluble in water.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Measurement of [Ca2+]i in whole cell suspensions using fura-2: R.A. Hirst, et al.; Methods Mol. Biol. 312, 37 (2006), Abstract;

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