Replaces Prod. #: ALX-620-005
Fura-2 acetomethoxy (AM) is one of the most widely used calcium indicators for homogenous quantitative dual wavelength ratiometric cell measurements. Fura-2 AM is particularly useful for digital imaging microscopy. It is less susceptible to photobleaching than Indo-1. The probe is excited only by UV light, which results in significantly less interference by visible wavelength excitable fluorescent compounds. One application of the probe is as a useful counter screen tool for GPCR and calcium channel hits identified using calcium indicators excited at 488nm in primary screens. Fura-2 AM can be easily loaded into cells by passive incubation. Wavelength Maxima: Excitation 370nm, Emission 476nm
Product Details
| Alternative Name: | 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester |
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| Formula: | C44H47N3O24 |
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| MW: | 1001.9 |
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| CAS: | 108964-32-5 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Off-white to yellow solid. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
TRPM3 Is Expressed in Afferent Bladder Neurons and Is Upregulated during Bladder Inflammation: M. Vanneste, et al.; Int. J. Mol. Sci.
23, 107 (2022),
Abstract;
Measurement of [Ca2+]i in whole cell suspensions using fura-2: R.A. Hirst, et al.; Methods Mol. Biol.
312, 37 (2006),
Abstract;
Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source: G. McConnell, et al.; Opt. Lett.
28, 1742 (2003),
Abstract;
