Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Fluo-3 (ultra pure)

Calcium dye
ENZ-52005 1 mg 198.00 USD
Do you need bulk/larger quantities?
Fluo-3 is widely used in flow cytometry, confocal laserscanning microscopy and fluorescence spectroscopy. The probe has proved particularly valuable for determining GPCR function via cell-based high-throughput screening. Fluo-3 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Once inside the cell, Fluo-3 becomes fluorescent, in the presence of free Ca (II). Fluo-3 is a long wavelength calcium probe (488 nm excitable). The longer wavelength of absorption is convenient for minimizing photodamage in cells. Wavelength Maxima: Excitation 506nm, Emission 526nm

Product Specification

Alternative Name:1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid . 5NH3
Purity:≥95% (HPLC)
Solubility:Soluble in DMSO.
Long Term Storage:-20°C
Use/Stability:Stable for at least one year after receipt when stored as recommended.
Handling:Protect from light. Keep cool and dry.
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Please mouse over

Product Literature References

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux: S. Bailey & P.J. Macardle; J. Immunol. Methods 311, 220 (2006), Abstract;
A laser scanning confocal microscopy method. Simultaneous detection of intracellular Ca2+ and apoptosis using Fluo-3 and Hoechst 33342: T. Zhang, et al.; Anal. Quant. Cytol. Histol. 22, 93 (2000), Abstract;

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Calcium Dye
By catalog section:


Technical Service
Customer Service

Related Literature

Live Cell Analysis
Live Cell Analysis
Download as PDF

All new literature pieces

Recommend this page

For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,