Calcein Blue (ultra pure)

Calcium dye



ENZ-52003	250 mg	81.00 USD

Calcein Blue has similar calcium-binding properties as calcein. Like calcein, it is typically used for metallochromic titration of metal ions with EDTA and demonstrates enhanced fluorescence upon binding Ca (II), Zn (II) or La (II). Binding of Mn (II), Fe (II), Co (II), Ni (II) and Cu (II) results in a reduction in fluorescence. In addition, the probe is also used as a reference standard for calcein AM. The probe also finds application in cell-based assays for measuring mineralized-tissue formation by cultured osteoblastic cells.

Alternative Name:	4-Methylumbelliferone-8-methyliminodiacetic acid

Formula:	C₁₅H₁₅NO₇

MW:	321.3

Purity:	≥95% (HPLC)

CAS:	54375-47-2

RTECS:	259-129-8

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Hazard:	IRRITANT.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue: T. Goto, et al.; Biomaterials 24, 3885 (2003), Abstract;

Fluorimetric determination of calcium in serum with calcein blue: K.A. Matsoukas & M.A. Demertzis; Analyst 113, 251 (1988), Abstract;