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Calcein AM (ultra pure)

Calcium dye
 
ENZ-52002 1 mg 135.00 USD
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Replaces Prod. #: ALX-610-026

Calcein acetomethoxy (AM), a derivative of calcein, is useful for differentiating between live and dead cells. Calcein AM readily passes through the cellular plasma membrane. Once inside, esterases cleave the AM groups yielding the more hydrophilic calcein, trapped inside the cell. The loss of the acetomethoxy group also enables calcein to readily bind intracellular calcium; resulting in a strong yellowish-green fluorescence. As dead cells lack cytoplasmic esterases, fluorescence is demonstrated exclusively in live cells, making the probe useful for determining cell viability. Calcein-AM exhibits low cytotoxicity and does not significantly affect cellular functions such as proliferation or chemotaxis. Viability assays using calcein correlate well with other assays, such as 51Cr-release. Wavelength Maxima: Excitation 495nm, Emission 515nm

Product Specification

Alternative Name:3\',6\'-Di(O-acetyl-2\',7\'-bis[N,N-bis(carboxymethyl)-aminomethyl]fluorescein tetraacetoxymethyl ester
 
Formula:C46H46N2O23
 
MW:994.9
 
CAS:148504-34-1
 
Purity:≥95% (HPLC)
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

Dual staining assessment of Schwann cell viability within whole peripheral nerves using calcein-AM and ethidium homodimer: P. Decherchi, et al.; J. Neurosci. Methods 71, 205 (1997), Abstract;

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