Calcein AM (ultra pure)

Calcium dye



ENZ-52002	1 mg	239.00 USD

Calcein acetomethoxy (AM), a derivative of calcein, is useful for differentiating between live and dead cells. Calcein AM readily passes through the cellular plasma membrane. Once inside, esterases cleave the AM groups yielding the more hydrophilic calcein, trapped inside the cell. The loss of the acetomethoxy group also enables calcein to readily bind intracellular calcium; resulting in a strong yellowish-green fluorescence. As dead cells lack cytoplasmic esterases, fluorescence is demonstrated exclusively in live cells, making the probe useful for determining cell viability.

Alternative Name:	3\',6\'-Di(O-acetyl-2\',7\'-bis[N,N-bis(carboxymethyl)-aminomethyl]fluorescein tetraacetoxymethyl ester

Formula:	C₄₆H₄₆N₂O₂₃

MW:	994.9

Purity:	≥95% (HPLC)

CAS:	148504-34-1

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Please mouse over

Product Literature References

Dual staining assessment of Schwann cell viability within whole peripheral nerves using calcein-AM and ethidium homodimer: P. Decherchi, et al.; J. Neurosci. Methods 71, 205 (1997), Abstract;