Calcein (ultra pure)

Calcium dye



ENZ-52001	100 mg	81.00 USD

Calcein is a fluorescent calcium indicator that emits a yellowish-green fluorescence under acidic conditions and is minimally fluorescent under basic conditions. Calcein also fluoresces in the presence of certain metal cations such as Al (III), Ba (II), Cu (II), Mg (II), Hg (II) and Zn (II) under basic conditions. Therefore, calcein can be used for direct fluorimetric titration of these heavy metal ions as well as Ca (II). Calcein self-quenches at concentrations above 100 mM and is slightly water soluble.

Alternative Name:	3,3\'-bis[N,N-bis(Carboxymethyl)aminomethyl]fluorescein

MW:	622.5

Purity:	≥90% (HPLC)

CAS:	1461-15-0

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

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Product Literature References

Selective ultratrace detection of Al(III) and Ga(III) complexed with a calcein isomer by capillary zone electrophoresis with laser-induced fluorescence detection. Comparison of emissive polyaminocarboxylates as derivatizing ligands: S. Saito, et al.; J. Chromatogr. A 1140, 230 (2007), Abstract;

Flow-injection fluorimetric determination of mercury(II) with calcein: A.G. Lista, et al.; Talanta 50, 881 (1999), Abstract;