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MITO-ID® Extracellular O2 Sensor Probe

Reagent used in high-throughput assays to monitor mitochondrial function and respiration rates, through the kinetic measurement of extracellular oxygen consumption.
 
ENZ-51047 1 Vial 290.00 USD
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  • For use on standard fluorescence plate readers (96 or 384 well format), for high-throughput analysis.
  • Direct real time kinetic analysis of extracellular oxygen consumption rates (OCR) as a measure of cellular respiration and mitochondrial function.
  • Component in Mito-ID® Extracellular O2 Sensor Kit (Prod. No. ENZ-51044) and Mito-ID® Extracellular O2 Sensor Kit (High Sensitivity) (Prod. No. ENZ-51045)
MITO-ID® Extracellular O2 Sensor Probe is a phosphorescent oxygen sensitive reagent enabling high throughput and real time oxygen consumption readout. Used as the component of an oxygen consumption assay, MITO-ID® Extracellular O2 Sensor Probe is quenched by oxygen, through molecular collision, and thus the amount of fluorescence signal is inversely proportional to the amount of extracellular oxygen in the sample. The addition of a suitable oil layer, as contained in MITO-ID® Extracellular O2 Sensor Kit (Prod. No. ENZ-51044) and MITO-ID® Extracellular O2 Sensor Kit (High Sensitivity) (Prod. No. ENZ-51045), is required to limit back diffusion of ambient oxygen. The flexible plate reader format, allows multiparametric or multiplex combination with a range of other reagents.

Product Specification

Absorbance maximum:340nm (optimal)
 
Emission maximum:630nm-680nm
 
Appearance:Dry solid.
 
Solubility:Soluble in nuclease free water or media.
 
Applications:Fluorescent detection, HTS
 
Application Notes:For the analysis of extracellular oxygen consumption rates (OCR), as a measure of cellular respiration and mitochondrial function.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Handling:Prior to use, centrifuge briefly to collect all contents. Avoid exposure to light. Reconstituted probe should be stored at -20°C and used within one month.
 
Protocol:See MITO-ID® Extracellular O2 Sensor Kit (Prod. No. ENZ-51044) and MITO-ID® Extracellular O2 Sensor Kit (High Sensitivity) (Prod. No. ENZ-51045) for protocols.
 
ENZ-51047 ex em
Excitation and Emission spectra of oxygen-sensitive probe MITO-ID® Extracellular O2 Sensor Probe. Left panel shows normalized excitation (EX 360-400nm; Peak 380nm). Right panel shows emission (Em 630–680nm; Peak 650nm) in oxygenated and deoxygenated conditions.
Please mouse over
ENZ-51047 ex em

Product Literature References

Adipogenic role of alternatively activated macrophages in β-adrenergic remodeling of white adipose tissue: Y.H. Lee, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 310, R55 (2016), Abstract;

General Literature References

High throughput fluorescence assays for the measurement of mitochondrial activity in intact human neuroblastoma cells: A.J. Woollacott & P.B. Simpson; J. Biomol. Screen 6, 413 (2001), Abstract;
A New Method for the Cytofluorometric Analysis of Mitochondrial Membrane Potential Using the J-Aggregate Forming Lipophilic Cation 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine Iodide (JC-1): A. Cossarizza, et al.; Biochem. Biophys. Res. Commun. 197, 40 (1993), Abstract;
Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate forming lipophilic cation JC-1: S.T. Smiley, et al.; PNAS 88, 3671 (1991), Full Text

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