ProteoStat^®Protein aggregation standards for microplates

As with any protein assay, different protein aggregates will elicit slightly greater or lesser fluorescence intensity response based upon their inherent amino acid composition and sequence. It is recommended that a standard curve be prepared each time the ProteoStat^® Protein Aggregation assay (Prod. No. ENZ-51023) is performed.

The ProteoStat^® Protein Aggregation standards contain stabilized, high-quality reference samples for generating an accurate standard curve. The standard curve is formulated from different amounts of aggregated IgG, combined with monomeric IgG. Once reconstituted, the samples contain 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, 0.39%, 0.20% and 0% aggregated protein, while the total protein concentration of each standard is maintained at 1mg/ml. The total protein concentration of the IgG standard has been calibrated by direct comparison to an internal protein standard, in order to ensure lot-to-lot consistency and accuracy. Orthogonal characterization methods, including laser-based nanoparticle tracking and micro-flow imaging, have been employed to validate monomer integrity during manufacturing.