Lyso-ID^® Green detection kit for microscopy



ENZ-51034-K500	1 Kit	213.00 USD

The Lyso-ID^® Green detection kit contains an acidic organelle-selective dye suitable for live-cell staining. Conventional fluorescent stains for acidic organelles, such as Acridine Orange (Prod. No. ENZ-52405), form metachromatic artifacts that interfere with multicolor imaging applications. Lyso-ID^® Green dye generates emission profiles that can be multiplexed with other fluorophores. The dye accumulates in acidic compartments, such as endosomes, lysosomes, and secretory vesicles.

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Figure 1: Lyso-ID® Green dye is a cell-permeant fluorescent probe that selectively associates with lysosomes and other acidic organelles (A). Cells pre-treated for 20 hours with weakly basic cell-permeant compounds, such as chloroquine, show dramatic increases in lysosome-like vesicle number and volume (B). Nuclei are counter-stained with Hoechst 33342 in the images.

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Product Specification

Quantity:	500 assays

Quality Control:	A sample from each lot of Lyso-ID^® Green detection kit is used to stain HeLa cells using the procedures described in the user manual. Analyzed by microscopy, the stained cells exhibit dramatic increase in lysosome-like vesicle number and volume in the chloroquine-treated HeLa cells.

Kit/Set Contains:	Lyso-ID^® Green detection reagent, 50μlHoechst 33342 nuclear staining, 50μlChloroquine control, 7.5μmol10x Assay buffer, 15ml

Application:	Specifically designed for use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Use/Stability:	With proper storage, the kit components are stable for 1 year from the date of receipt.

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	The Lyso-ID^® Green detection kit is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

General Literature References

Photoconversion of Lysotracker Red to a green fluorescent molecule: E.C. Freundt, et al.; Cell Res. 17, 956 (2007), Abstract;

Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes: J.K. Jaiswal, et al.; PNAS 104, 14151 (2007), Abstract;

Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles: F. Nadrigny, et al.; Biophys. J. 93, 969 (2007), Abstract;

Disruptive effect of chloroquine on lysosomes in cultured rat hepatocytes: A. Michihara, et al.; Biol. Pharm. Bull. 28, 947 (2005), Abstract;

Photo-oxidative disruption of lysosomal membranes causes apoptosis of cultured human fibroblasts: U.T. Brunk, et al.; Free Radic. Biol. Med. 23, 616 (1997), Abstract;