Organelle-ID RGB™ III assay kit



ENZ-51032-K100	1 Kit	319.00 USD

Assay Golgi and ER dynamics in live cells
Highly resistant to photo-bleaching, concentration quenching and photoconversion
Identify modulators of secretory membrane traffic and organelle integrity

Compared with other commercially available dyes for labeling Golgi bodies, the green dye component of the Organelle-ID RGB™ III detection reagent is more faithfully localized to the Golgi apparatus, with minimal staining of the endoplasmic reticulum. The red dye component of the reagent stains the endoplasmic reticulum with high fidelity and is specifically designed for use with green fluorescing probes. Organelle-ID RGB™ III assay kit is validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.

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Figure 1: Organelle-ID RGB™ III kit staining of MDCK epithelial cells. The ER-ID™ Red, Golgi-ID™ Green and Hoechst 33342 (blue) dyes highlight their respective subcellular targets with high dependability.

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Product Specification

Quantity:	100 assays. For detection of the Golgi apparatus, endoplasmic reticulum and nucleus by microscopy.

Quality Control:	A sample from each lot of Organelle-ID RGB™ III assay kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivities of the red, green and blue stains are evident.

Kit/Set Contains:	Organelle-ID RGB™ Reagent III (lyophilized), 1 vial 10X Assay Buffer 1, 15 ml 50X Assay Buffer 2, 1.2 ml

Application:	The Organelle-ID RGB™ III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining. The kit should also be suitable for identifying Golgi body and endoplasmic reticulum perturbing agents and thus can be a useful tool for examining the transport and recycling of molecules from the Golgi apparatus to endoplastic reticulum in cellular secretory pathways.

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Use/Stability:	With proper storage, the kit components are stable for 1 year from date of receipt.

Handling:	Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	The Organelle-ID RGB™ III Assay Kit is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required. This product is manufactured and sold by ENZO LIFE SCIENCES, INC. for research use only by the end-user in the research market and is not intended for diagnostic or therapeutic use. Purchase does not include any right or license to use, develop or otherwise exploit this product commercially. Any commercial use, development or exploitation of this product or development using this product without the express prior written authorization of ENZO LIFE SCIENCES, INC. is strictly prohibited.

General Literature References

The Golgi apparatus as a crossroads in intracellular traffic: A. Mironov & M. Pavelka; The Golgi Apparatus: State of the art 110 years after Camillo Golgis Discovery Mironov and Pavelka, Eds., Springer, New York, (2008), Book, Abstract;

Golgi-disturbing agents: A. Dinter & E.G. Berger; Histochem. Cell. Biol. 109, 571 (1998), Abstract;

Fluorescent conjugates of brefeldin A selectively stain the endoplasmic reticulum and Golgi complex of living cells: Y. Deng, et al.; J. Histochem. Cytochem. 43, 907 (1995), Abstract;