ProteoStat^® Protein aggregation assay

Quantitative Detection of Protein Aggregates from Visible to Subvisible Particles



ENZ-51023-KP002	1 Kit	425.00 USD

A simple, sensitive, homogenous fluorescent microplate assay
Extensively benchmarked with IgG
Optimize buffers and excipients for protein formulation
Performs with a wide pH and ionic strength range
ProteoStat^® Protein Aggregation Standards ... easy to use - simply add water! Developed for use with ProteoStat^® Protein Aggregation kit

ProteoStat^® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures. Relative to conventional protein aggregation detection dyes, such as Thioflavin T, the Enzo ProteoStat^® detection reagent can detect aggregates from a broader range of proteins, yields a much brighter signal, provides at least 2 orders of magnitude linear dynamic range and offers superior performance across a broad range of pH values (4~10) and buffer compositions.

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Product Specification

Alternative Name:	Amyloid

Quantity:	2 x 96-well plates

Quality Control:	A sample of ProteoStat^® Protein aggregation assay was used to assay (1) 20 µM aggregated lysozyme, (2) 20 µM native lysozyme; and (3) mixtures of 5% aggregate. The Z’ factor is greater than 0.5 and the 5% aggregate signal is greater than 3 standard deviations above the no-aggregate control.

Kit/Set Contains:	ProteoStat^® detection reagent, 22 µl ProteoStat^® positive control, 300 µg, Aggregate ProteoStat^® negative control, 300 µg, Monomer 10X ProteoStat^® assay buffer, 15 ml

Application:	This kit has been specifically designed for monitoring of protein aggregate formation in solution.

Long Term Storage:	-80°C

Use/Stability:	With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer or -80°C for longer term storage.

Background / Technical Information:	Enzo and ProteoStat are trademarks of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.