Nuclear-ID™ Green chromatin condensation detection kit for fluorescence microscopy and flow cytometry



ENZ-51021-K200	1 Kit	240.00 USD

Enzo Life Sciences’ Nuclear-ID™ Green Chromatin Condensation Detection Kit provides a rapid and convenient assay for one of the prominent hallmarks of apoptosis, nuclear condensation. The kit contains a DNA intercalating dye that brightly stains the condensed chromatin of apoptotic cells, but only dimly stains the normal chromatin of live cells. This staining pattern makes it possible to distinguish between healthy and apoptotic cell populations by fluorescence microscopy or flow cytometry.

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Product Specification

Quantity:	200 assays

Quality Control:	1. Absorption peak of Nuclear-ID™ Green dye: λ_max = 507 ± 4 nm 2. % purity of Nuclear-ID™ Green dye by HPLC: ≥93% 3. A sample from each lot of Nuclear-ID™ Green Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. The following results are obtained: (a) Untreated control cells: Viable cells: >85%; Apoptotic cell population: <15% (b) Apoptosis inducer treated cells: Viable cells: >60%; Apoptotic cell population: <30% (c) Mean fluorescence of apoptotic cells / mean fluorescence of viable cells: >40

Kit/Set Contains:	Nuclear-ID™ Green Cell Cycle Detection Reagent, 100 µL Apoptosis inducer (Staurosporine), 50nmoles 10X Assay Buffer, 30 mL

Application:	This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.

Shipping:	SHIPPED ON DRY ICE

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Handling:	Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	Please click here for the comprehensive manual. The Nuclear-ID™ Green Cell Cycle Analysis Kit is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

General Literature References

Multiparametric analysis of apoptosis by flow and image cytometry. W. G. Telford et al. Methods Mol. Biol. 263 141 (2004)

Nuclear condensation and free radical scavenging: a dual mechanism of bisbenzimidazoles to modulate radiation damage to DNA. U. Tawar et al. Mol Cell Biochem. 305 221 (2007) Abstract

Three distinct stages of apoptotic nuclear condensation revealed by time-lapse imaging, biochemical and electron microscopy analysis of cell-free apoptosis. S. Toné et al. Exp Cell Res. 313 3635 (2007) Abstract Full Text