Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

GFP-CERTIFIED® FLUOFORTE® Calcium assay kit

A brighter, more robust and GFP-compatible (orange dye) fluorescent assay for calcium mobilization
 
ENZ-51020-KP010 1 Kit 444.00 USD
 
ENZ-51020-KP100 1 Kit 3,188.00 USD
Do you need bulk/larger quantities?
 
  • Monitor calcium mobilization in GFP-expressing cell lines
  • The brightest and most sensitive red-shifted fluorescent Ca2+ indicator
  • Provides comparable EC50 and IC50 values as obtained using Rhod-4 AM and Fluo-4 AM
  • Larger assay window and higher Z' factor values, allowing measurements of challenging cell lines and receptors
Enzo Life Sciences’ GFP-CERTIFIED® FLUOFORTE® Calcium Assay Kit (high-throughput) provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Relative to other commercially available dyes, the GFP-CERTIFIED® FLUOFORTE® dye is the brightest and most sensitive fluorescent calcium indicator. The kit provides a homogeneous mix-and-read, no-wash calcium mobilization assay. The homogenous cell-based assay for calcium offers fewer steps, lower variability and an easier protocol for adherent and non-adherent cell lines. In addition, it requires neither a washing step, nor exogenous addition of a quencher dye, which could adversely effect receptor-ligand interaction kinetics.
ENZ-51020 Fig3
Histamine H1 receptors: HeLa cells were seeded overnight at 40,000 cells per 100 µL per well in a 96-well black wall/clear bottom microplate. The cells were incubated with 100 µL of GFP-CERTIFIED® FLUOFORTE® reagent or Rhod-2 AM dye for 1 hour at 37°C. Histamine (20µL/well) was added using a BioTek two-syringe pump dispenser to achieve the final indicated concentrations. No significant difference in EC50 of Histamine for GFP-CERTIFIED® FLUOFORTE® and Rhod2-AM dye was observed. GFP-CERTIFIED® FLUOFORTE® reagent generated higher intensity signal and a larger assay window.
ENZ-51020 FLUOFORTE vs RHOD-2
U-2 OS cells were incubated with (A) GFP-CERTIFIED® FLUOFORTE® AM dye or (B) Rhod-2 AM dye. Cells were washed and treated with a final concentration of 100nM ATP and imaged immediately. GFP-CERTIFIED® FLUOFORTE® AM dye exhibits brighter fluorescence intensity than Rhod-2 AM.
ENZ 51020 spectra
Please mouse over
ENZ-51020 Fig3 ENZ-51020 FLUOFORTE vs RHOD-2 ENZ 51020 spectra

Product Specification

Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
 
Application Notes:The GFP-CERTIFIED® FLUOFORTE® Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.
 
Quality Control:% purity of Reagent A (GFP-CERTIFIED® FLUOFORTE® dye) by HPLC is ≥95%.
 
Quantity:ENZ-51020-KP010: for 10 x 96-well plates
ENZ-51020-KP100: for 100 x 96-well plates
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Kit/Set Contains:Starter pack (Prod. No. ENZ-51020-KP010):
Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 1 vial
Reagent B (dye efflux inhibitor), 10ml
Reagent C (Hanks’ buffer with 20mM HEPES, 100ml)
Sufficient for 10 x 96-well plates

High-throughput (Prod. No. ENZ-51020-KP100):
Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 10 vials
Reagent B (dye efflux inhibitor), 10 x 10ml
Sufficient for 100 x 96-well plates
 
Technical Info/Product Notes:The GFP-CERTIFIED® FLUOFORTE® Calcium assay kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.
 

Product Literature References

Gα13/PDZ-RhoGEF/RhoA signaling is essential for gastrin-releasing peptide receptor-mediated colon cancer cell migration: M. Patel, et al.; Mol. Pharmacol. 86, 252 (2014), Application(s): Microplate reader using Caco-2 cells, Abstract; Full Text
The N-terminally truncated µ3 and µ3-like opioid receptors are transcribed from a novel promoter upstream of exon 2 in the human OPRM1 gene: S. Andersen, et al.; PLoS One. 8, e71024 (2013), Application(s): Microplate reader using HEK293 cells, Abstract; Full Text
Leukotriene C4 induces migration of human monocyte-derived dendritic cells without loss of immunostimulatory function: J. Dannull, et al.; Blood 119, 3113 (2012), Application(s): Intracellular calcium release in human monocyte derived dendritic cells with Tecan Infinite plate reader, Abstract; Full Text

General Literature References

Novel Fluo-4 analogs for fluorescent calcium measurements: V.V. Martin, et al. ; Cell Calcium 36, 509 (2004), Abstract;
Calcium measurements in perfused mouse heart: quantitating fluorescence and absorbance of Rhod-2 by application of photon migration theory: C. Du, et al.; Biophys. J. 80, 549 (2001), Full Text
Rhod-2 based measurements of intracellular calcium in the perfused mouse heart: cellular andsubcellular localization and response to positive inotropy: G.A. MacGowan, et al.; J. Biomed. Opt. 6, 23 (2001), Abstract;
Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41: M. do Céu Monteiro, et al.; Cytometry 35, 302 (1999), Abstract;
Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3: C. Perez-Terzic, et al.; Cell Calcium 21, 275 (1997), Abstract;
Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets: R. Greimers, et al.; Cytometry 23, 205 (1996), Abstract;

Related Products

FLUOFORTE® Reagent 

Calcium dye
≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-52014 5x50 µg 245.00 USD
Do you need bulk/larger quantities?
 

FLUOFORTE® Reagent 

Calcium dye
≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-52015 1 mg 294.00 USD
Do you need bulk/larger quantities?
 

FLUOFORTE® Calcium assay kit  

A Brighter, More Robust Fluorescent Assay for Calcium Mobilization
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51016 100x96 tests 2,300.00 USD
Do you need bulk/larger quantities?
 

FLUOFORTE® Calcium assay kit  

A Brighter, More Robust Fluorescent Assay for Calcium Mobilization
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51017 10x96 tests 330.00 USD
Do you need bulk/larger quantities?
 

GFP-CERTIFIED® FLUOFORTE® Reagent 

Calcium dye
≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-52016-5C50 5x50 µg 250.00 USD
 
ENZ-52016-M001 1 mg 300.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
Calcium
By biological activity:
Calcium Detection kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Predictive Toxicology
Predictive Toxicology
Download as PDF

Scientific Posters
Quencher Dyes Can Alter the Pharmacokinetic Response Profi les of Living Cells in Fluorescence-based Calcium Mobilization Assays
Quencher Dyes Can Alter the Pharmacokinetic Response Profi les of Living Cells in Fluorescence-based Calcium Mobilization Assays
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,