GFP-Certified™ FluoForte™ Calcium assay kit for microplates



ENZ-51020-KP010	1 Kit	575.00 USD

ENZ-51020-KP100	1 Kit	3'450.00 USD

Monitor calcium mobilization in GFP-expressing cell lines
The brightest and most sensitive red-shifted fl uorescent Ca2+ indicator
Provides comparable EC50 and IC50 values as obtained using Rhod-4 AM and Fluo-4 AM
Larger assay window and higher Z' factor values, allowing measurements of challenging cell lines and receptors

Enzo Life Sciences’ GFP-Certified™ FluoForte™ Calcium Assay Kit (high-throughput) provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Relative to other commercially available dyes, the GFP-Certified™ FluoForte™ dye is the brightest and most sensitive fluorescent calcium indicator. The kit provides a homogeneous mix-and-read, no-wash calcium mobilization assay. The homogenous cell-based assay for calcium offers fewer steps, lower variability and an easier protocol for adherent and non-adherent cell lines.

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Product Specification

Quantity:	ENZ-51020-KP010: for 10 x 96-well plates ENZ-51020-KP100: for 100 x 96-well plates

Quality Control:	% purity of Reagent A (GFP-Certified™ FluoForte™ dye) by HPLC is ≥95%.

Kit/Set Contains:	Starter pack (Prod. No. ENZ-51020-KP010): Reagent A (lyophilized GFP-Certified™ FluoForte™ dye), 1 vial Reagent B (dye efflux inhibitor), 10ml Reagent C (Hanks’ buffer with 20mM HEPES, 100ml) Sufficient for 10 x 96-well plates High-throughput (Prod. No. ENZ-51020-KP100): Reagent A (lyophilized GFP-Certified™ FluoForte™ dye), 10 vials Reagent B (dye efflux inhibitor), 10 x 10ml Sufficient for 100 x 96-well plates

Application:	The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

Shipping:	SHIPPED ON DRY ICE

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Handling:	Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	For the Manual please click here. The GFP-Certified™ FluoForte™ Calcium Assay Kit is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

General Literature References

Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets R. Greimers, et al. Cytometry 23 205 (1996) Abstract

Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3 C. Perez-Terzic, et al. Cell Calcium 21 275 (1997) Abstract

Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41 M. do Céu Monteiro, et al. Cytometry 35 302 (1999) Abstract

Calcium measurements in perfused mouse heart: quantitating fluorescence and absorbance of Rhod-2 by application of photon migration theory C. Du, et al. Biophys. J. 80 549 (2001) Full Text

Rhod-2 based measurements of intracellular calcium in the perfused mouse heart: cellular andsubcellular localization and response to positive inotropy G.A. MacGowan, et al. J. Biomed. Opt. 6 23 (2001) Abstract

Novel Fluo-4 analogs for fluorescent calcium measurements V.V. Martin, et al. Cell Calcium 36 509 (2004) Abstract