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Easy staining protocol, simply add the dye and analyze by flow cytometry
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Excited using common 488 nm laser source, generating a green ~530 nm emission
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No permeabilization step or RNase treatment necessary
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Cell cycle results independent of incubation time, temperature, dye and cell concentrations
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Provides DNA content information in live or fixed cells
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Monitor changes in cell cycle dynamics arising from drug treatment or other perturbations
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Performance validated using a wide range of cell densities
NUCLEAR-ID® Green Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. The kit is suitable for (1) determining the percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as to quantify cells in the sub-G1 phase prior to apoptosis, and (2) DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels. The kit contains sufficient reagents for approximately 100 flow cytometry assays. A control cell cycle perturbation agent, Nocodazole, is provided for monitoring changes in cell cycle dynamics. Potential applications for live-cell studies are in the determination of cellular DNA content and cell cycle distribution for the detection of variations in growth patterns, for monitoring apoptosis, and for evaluating tumor cell behavior and suppressor gene mechanisms.
Product Details
| Applications: | Flow Cytometry, Fluorescence microscopy
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| Application Notes: | This kit is suitable for DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels. |
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| Quality Control: | - Absorption peak of NUCLEAR-ID® Green dye: λmax = 507 ± 4 nm
% purity of NUCLEAR-ID® Green dye by HPLC: ≥93% A sample from each lot of NUCLEAR-ID® Green Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. Cells with Nocodazole gave %G2 value of >50%. Untreated cells gave the following results: (a) G0/G1 peak CV < 15%; (b) %G1 > 35%; (c) %G2 < 30%; and (d) G2/G1 ratio > 1.72.
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| Quantity: | 100 assays |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Shipping: | Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Contents: | NUCLEAR-ID® Green Cell Cycle Detection Reagent, 100 µl
Nocodazole Control, 10 µl
10X Assay Buffer, 15 ml |
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| Technical Info/Product Notes: | The NUCLEAR-ID® Green cell cycle analysis kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required . |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Transcriptomic and functional studies reveal miR-431-5p as a tumour suppressor in pancreatic ductal adenocarcinoma cells: O.P. Haugen, et al.; Gene
822, 146346 (2022),
Abstract;
Low‐intensity pulsed ultrasound stimulation promotes osteoblast differentiation through hedgehog signaling: K. Matsumoto, et al.; J. Cell. Biochem.
119, 4352 (2018),
Abstract;
Nifurtimox reduces N-Myc expression and aerobic glycolysis in neuroblastoma: K.M. Cabanillas Stanchi, et al.; Cancer Biol. Ther.
16, 1353 (2015),
Application(s): Flow cytometry using neuroblastoma cells,
Abstract;
Full Text
General Literature References
DNA measurement and cell cycle analysis by flow cytometry: R. Nunez; Curr. Issues Mol. Biol.
3, 67 (2001),
Abstract;
Methods in Cell Biology, Flow Cytometry: Z. Darzynkiewicz, H.A. Crissman and J.P. Robinson (editors and co-authors); Vol. I and II, (1994), Book,
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