United States
Nuclear-ID® Green cell cycle kit for flow cytometry
Cell Cycle Results Independent of Incubation Time, Temperature, Dye and Cell Concentrations
| ENZ-51014-100 | 1 Kit | 289.00 USD |  |
||
- Easy staining protocol, simply add the dye and analyze by flow cytometry
- Excited using common 488 nm laser source, generating a green ~530 nm emission
- No permeabilization step or RNase treatment necessary
- Cell cycle results independent of incubation time, temperature, dye and cell concentrations
- Provides DNA content information in live or fixed cells
- Monitor changes in cell cycle dynamics arising from drug treatment or other perturbations
- Performance validated using a wide range of cell densities
Nuclear-ID® Green Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. The kit is suitable for (1) determining the percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as to quantify cells in the sub-G1 phase prior to apoptosis, and (2) DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels.
Product Specification
| Quantity: | 100 assays |
| Quality Control: | 1. Absorption peak of Nuclear-ID® Green dye: λmax = 507 ± 4 nm 2. % purity of Nuclear-ID® Green dye by HPLC: ≥93% 3. A sample from each lot of Nuclear-ID® Green Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. Cells with Nocodazole gave %G2 value of >50%. Untreated cells gave the following results: (a) G0/G1 peak CV < 15%; (b) %G1 > 35%; (c) %G2 < 30%; and (d) G2/G1 ratio > 1.72. |
| Kit/Set Contains: | Nuclear-ID® Green Cell Cycle Detection Reagent, 100 µl Nocodazole Control, 10 µl 10X Assay Buffer, 15 ml |
| Application: | This kit is suitable for DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels. |
| Short Term Storage: | -20°C |
| Long Term Storage: | -80°C |
| Handling: | Avoid freeze/thaw cycles. Protect from light. |
| Background / Technical Information: | The Nuclear-ID™ Green Cell Cycle Analysis Kit is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
General Literature References
DNA measurement and cell cycle analysis by flow cytometry: R. Nunez; Curr. Issues Mol. Biol. 3, 67 (2001), Abstract;
Methods in Cell Biology, Flow Cytometry: Z. Darzynkiewicz, H.A. Crissman and J.P. Robinson (editors and co-authors); Vol. I and II, (1994), Book,
Product Toolbox
PRODUCT RESOURCES
Datasheet
MSDS
Manual
RELATED PRODUCTS
By catalog section:
By biological activity:
Nucleus Labeling kitDNA Detection kit
DNA Detection kit
PRODUCT SUPPORT
Technical ServiceBulk Quantity Quote
Customer Service
Related Literature
Catalogs
Genomics & Molecular Biology
Add to order list
 |
 03-May-12 |
| Download as PDF | |
Poster Presentations
Evaluating Small Molecule Cell Cycle and Chromatin Condensation Modulators Using a Novel Green Fluorescent DNA-Binding Probe: Potential Application to Compound Screening
Add to order list
 |
06-Apr-11 |
| Download as PDF | |
