Nuclear-ID™ Red cell cycle kit (GFP-Certified™) for flow cytometry



ENZ-51008-100	1 Kit	289.00 USD

Complete kit provides DNA content information in live, permeabilized or fixed cells
Performance validatedusing a wide range of cell densities
Monitors changes in cell cycle dynamicsarising from drug treatment or other perturbations
Excited using common laser sources generating far red / green emission
True multiplexed capability with additional probes and dyes
Easy staining protocol– simply add the dye and analyze by flow cytometry
Stringently manufactured to control and eliminate non-specific assay artifacts

Enzo Life Sciences’ GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. The kit is suitable for (1) determining the percentage of cells in a given sample that are in G₀/G₁, S and G₂/M phases, as well as to quantify cells in the sub-G₁ phase, and (2) DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels.

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Figure 1: Three-dimensional reconstruction of the spatial relationship between the green fluorescent protein-expressing (GFP-expressing) mitochondria and the nucleus using a structured illumination method, as implemented with the ApoTome from Carl Zeiss, Inc. This imaging method enabled creation of optical sections through the nucleus using a conventional fluorescence microscope, for improved resolution along the optical axis. The optical sections were then used to create a 3-D reconstruction of the nucleus, enabling the GFP-expressing mitochondria to be displayed in their proper spatial context.

Figure 2: Drug treatments with live cells inhibit cell cycle progression at different phases.

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Product Specification

Quantity:	100 assays

Quality Control:	1. Absorption peak of Nuclear-ID™ Red dye: λ_max = 566 ± 4 nm 2. % purity of Nuclear-ID™ Red dye by HPLC: ≥93% 3. A sample from each lot of GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. Cells with Nocodazole gave %G₂ value of >60%. Untreated cells gave the following results: (a) G₀/G₁ peak CV < 15%; (b) %G₁ > 35%; (c) %G₂ < 15%; and (d) G₂/G₁ ratio > 1.8.

Kit/Set Contains:	Nuclear-ID™ Red Cell Cycle Detection Reagent, 200 µL Nocodazole Control, 10 µL 10X Assay Buffer, 15 mL

Application:	Suitable for DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels..

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Use/Stability:	With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Handling:	Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	The GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. For an application note please click here.

Product Literature References

A cell-permanent dye for cell cycle analysis by flow and laser-scanning microplate cytometry: YueJun Xiang, et al.; Nature Methods 6, an2 (2009), Abstract;

General Literature References

DNA measurement and cell cycle analysis by flow cytometry: R. Nunez; Curr. Issues Mol. Biol. 3, 67 (2001), Abstract;

Methods in Cell Biology, Flow Cytometry: Z. Darzynkiewicz, H.A. Crissman and J.P. Robinson (editors and co-authors); Vol. I and II, (1994), Book,