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MITO-ID® Red detection kit (GFP-CERTIFIED®)

Photostable,  non-toxic and selective mitochondrial dye that stains regardless of membrane  potential
 
ENZ-51007-0100 100 tests 81.00 USD
 
ENZ-51007-500 500 tests 237.00 USD
Do you need bulk/larger quantities?
 
  • Mitochondria-selective dye stains live, detergent permeabilized and aldehyde fixed cells
  • Long wavelength red emission is easily multiplexed with common fluorescent dyes
  • Highly resistant to photobleaching and concentration quenching, for strong, consistent fluorescent signal
  • Highlights mitochondria regardless of the organelle’s membrane potential status
  • Stringently manufactured, to control and eliminate non-specific assay artifacts
MITO-ID® Red Detection Kit (GFP-CERTIFIED®) contains a proprietary membrane-permeable mitochondria-selective dye suitable for use with live-, detergent-permeabilized- and even aldehyde-fixed-cells. Unlike conventional dyes, such as DiOC6(3), JC-1, rhodamine 123 and tetramethylrhodamine ethyl ester, MITO-ID® Red dye highlights mitochondria regardless of their energetic state. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as, High Content Screening (HCS) platforms. The kit is useful for assessing mitochondrial morphology changes, estimating mitochondrial mass and co-localizing GFP-tagged proteins to the mitochondrial compartment. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with fluorescent probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties such as fluorescein and coumarin. A nuclear counterstain (Hoechst 33342) is provided to highlight this organelle as well. Wavelength maxima: MITO-ID® Red λex 558 nm, λem 690 nm; Hoechst 33342 λex 350 nm, λem 461 nm.
ENZ-51007 kit box
MITO-ID® Red detection kit (GFP-CERTIFIED®)
ENZ-51007 Fig2
The MITO-ID® Red dye selectively stains mitochondria of living cells and is relatively insensitive to mitochondrial membrane potential uncouplers of phosphorylation, such as CCCP (carbonyl cyanide 3-chlorophenylhydrazone) as well as ion-channel perturbing drugs, such as valinomycin.
ENZ-51007 Fig1
Composite fluorescence microscopy images of HeLa cells (40X objective lens). Cells were stained with MITO-ID® Red dye for 15 minutes. Nuclei were counter-stained with Hoechst 33342 dye.
ENZ-51007 Fig3
HeLa-TurboGreen-mitochondria cells (HeLa-mitoGFP, MarinPharm GmbH, Luckenwalde, Germany) stained with MITO-ID® Red and Hoechst 33342 (blue) dyes. MITO-ID® Red co-localizes with the EGFP-cytochrome C oxidase signal (yellow signal), demonstrating selectivity for mitochondria. Note that mitochondria in cells no longer expressing the GFP-tagged protein appear red in the composite image.
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ENZ-51007 kit box ENZ-51007 Fig2 ENZ-51007 Fig1 ENZ-51007 Fig3

Product Specification

Applications:Fluorescence microscopy, Fluorescent detection
 
Application Notes:For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.
 
Quality Control:
  1. Absorption peak of MITO-ID® Red Detection Reagent: λmax= 550 ± 7 nm
  2. % purity of MITO-ID® Red Detection Reagent by HPLC: ≥93%
  3. A sample from each lot of GFP-CERTIFIED® MITO-ID® Red Mitochondrial Detection Kit is used to stain HeLa cells, expressing GFP-cytochrome C oxidase, using the procedures described in the user manual. The selectivity of the MITO-ID® Red dye is evident as shown by the co-localization with a GFP-cytochrome C oxidase fusion expressed in the HeLa cells.
 
Quantity:For ENZ-51007-500, 500 assays
For ENZ-51007-0100, 100 assays
 
Use/Stability:With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or –80°C for longer term storage.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Kit/Set Contains:MITO-ID® Red Detection Reagent
Hoechst 33342 Nuclear Stain
10X Assay Buffer
 
Technical Info/Product Notes:Application Note:
Towards Understanding the Molecular Basis of Parkinson’s Disease: Cell-based Model of Mitophagy and Aggresome Accumulation

 
 

Product Literature References

Functional characterization of the first filamentous fungal tRNA-isopentenyltransferase and its role in the virulence of Claviceps purpurea: J. Hinsch, et al.; New Phytol. 211, 980 (2016), Abstract;
Mitochondrial targeting increases specific activity of a heterologous valine assimilation pathway in Saccharomyces cerevisiae: K.V. Solomon, et al.; Metab. Eng. Commun. 3, 68 (2016), Application(s): Protein localization,
Effects of two fullerene derivatives on monocytes and macrophages: S. Pacor, et al.; Biomed. Res. Int. 2015, Article ID 915130 (2015), Application(s): Confocal microscopy on monocytes and macrophages, Abstract; Full Text
Induction of androgen formation in the male by a TAT-VDAC1 fusion peptide blocking 14-3-3ɛ protein adaptor and mitochondrial VDAC1 interactions: Y. Aghazadeh, et al.; Mol. Ther. 22, 1779 (2014), Abstract;
Protein modifications regulate the role of 14-3-3γ adaptor protein in cAMP-induced steroidogenesis in MA-10 Leydig cells: Y. Aghazadeh, et al.; J. Biol. Chem. 289, 26542 (2014), Abstract;
Mitochondrial fission induced by platelet-derived growth factor regulates vascular smooth muscle cell bioenergetics and cell proliferation: J.K. Salabei, et al.; Redox Biol. 1, 542 (2013), Application(s): Detection by confocal microscopy, Abstract; Full Text
Utilization of fluorescent probes for the quantification and identification of subcellular proteomes and biological processes regulated by lipid peroxidation products: T.D. Cummins, et al.; Free Radic. Biol. Med. 59, 56 (2013), Application(s): Detection by confocal microscopy, Abstract; Full Text
Ectopic ATP synthase blockade suppresses lung adenocarcinoma growth by activating the unfolded protein response: H.Y. Chang, et al.; Cancer Res. 72, 4696 (2012), Abstract; Full Text
High-mobility group A1 protein inhibits p53-mediated intrinsic apoptosis by interacting with Bcl-2 at mitochondria: F. Esposito, et.al; Cell Death Dis. 3, e383 (2012), Application(s): Mitochondrial staining in HEK293cells transfected with EGFP-HMGA1b, Abstract; Full Text
Hormone-induced 14-3-3γ adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells: Y. Aghazadeh, et al.; J. Biol. Chem. 287, 15380 (2012), Application(s): Detection of mitochondria in MA-10 mouse Leydig tumor cells using confocal microscopy, Abstract; Full Text
Intracellular Energetic Units regulate metabolism in cardiac cells: V. Saks, et al.; J. Mol. Cell. Cardiol. 52, 419 (2012), Application(s): Detection of mitochondria in cardiomyocytes using confocal microscopy, Abstract;
Opa3, a novel regulator of mitochondrial function, controls thermogenesis and abdominal fat mass in a mouse model for Costeff syndrome: T. Wells, et al.; Hum. Mol. Genet. 18, 4836 (2012), Application(s): Visualization of mitochondria in paraffin embedded sections of mouse brown adipose tissue with Mito-ID® Red detection kit., Abstract; Full Text
Cytometric assessment of mitochondria using fluorescent probes: C. Cottet-Rousselle, et al.; Cytometry A 79, 405 (2011), Abstract; Full Text
Mitochondria-cytoskeleton interaction: distribution of β-tubulins in cardiomyocytes and HL-1 cells: R. Guzun, et al.; Biochim. Biophys. Acta 1807, 458 (2011), Application(s): Detection of mitochondria in cardiomyocytes using fluorescence microscopy, Abstract;
The targeting of plasmalemmal ceramide to mitochondria during apoptosis: E.B. Babiychuk, et al.; PLoS One 6, e23706 (2011), Application(s): Detection of mitochondria in T cell and monocyte cell lines using fluorescence microscopy, Abstract; Full Text

General Literature References

Photoconversion of Lysotracker Red to a green fluorescent molecule: E.C. Freundt, et al.; Cell Res. 17, 956 (2007), Abstract;
Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles: F. Nadrigny, et al.; Biophys. J. 93, 969 (2007), Abstract;
Chloromethyl-X-rosamine (MitoTracker Red) photosensitises mitochondria and induces apoptosis in intact human cells: T. Minamikawa, et al.; J. Cell. Science 112, 2419 (1999), Abstract;
Chloromethyltetramethylrosamine (Mitotracker Orange) induces the mitochondrial permeability transition and inhibits respiratory complex I. Implications for the mechanism of cytochrome c release: L. Scorrano, et al; J. Biol. Chem. 274, 24657 (1999), Abstract;

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