Mito-ID^® Red detection kit (GFP-Certified™) for microscopy



ENZ-51007-500	1 Kit	213.00 USD

Mitochondria-selective dye stains live, detergent permeabilized and aldehyde fixed cells
Long wavelength red emission is easily multiplexed with common fluorescent dyes
Highly resistant to photobleaching and concentration quenching, for strong, consistent fluorescent signal
Highlights mitochondria regardless of the organelle’s membrane potential status
Stringently manufactured, to control and eliminate non-specific assay artifacts

Mito-ID^® Red Detection Kit (GFP-Certified™) contains a proprietary membrane-permeable mitochondria-selective dye suitable for use with live-, detergent-permeabilized- and even aldehyde-fixed-cells. Unlike conventional dyes, such as DiOC6(3), JC-1, rhodamine 123 and tetramethylrhodamine ethyl ester, Mito-ID^® Red dye highlights mitochondria regardless of their energetic state. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as, High Content Screening (HCS) platforms.

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Figure 1: Composite fluorescence microscopy images of HeLa cells (40X objective lens). Cells were stained with Mito-ID® Red dye for 15 minutes. Nuclei were counter-stained with Hoechst 33342 dye.

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Product Specification

Quantity:	500 assays

Quality Control:	1. Absorption peak of Mito-ID^® Red Detection Reagent: λ_max= 550 ± 7 nm 2. % purity of Mito-ID^® Red Detection Reagent by HPLC: ≥93% 3. A sample from each lot of GFP-Certified™ Mito-ID^® Red Mitochondrial Detection Kit is used to stain HeLa cells, expressing GFP-cytochrome C oxidase, using the procedures described in the user manual. The selectivity of the Mito-ID^® Red dye is evident as shown by the co-localization with a GFP-cytochrome C oxidase fusion expressed in the HeLa cells.

Kit/Set Contains:	Mito-ID^® Red Detection Reagent, 10 µL Hoechst 33342 Nuclear Staining, 50 µL 10X Assay Buffer, 15 mL

Application:	For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.

Long Term Storage:	-80°C

Use/Stability:	With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or –80°C for longer term storage.

Handling:	Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	Mito-ID^® Red Mitochondrial Detection Kit (GFP-Certified^®) is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Product Literature References

Cytometric assessment of mitochondria using fluorescent probes: C. Cottet-Rousselle, et al.; Cytometry A. Jun 79(6), 405 (2011), Abstract;

General Literature References

Photoconversion of Lysotracker Red to a green fluorescent molecule: E.C. Freundt, et al.; Cell Res. 17, 956 (2007), Abstract;

Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles: F. Nadrigny, et al.; Biophys. J. 93, 969 (2007), Abstract;

Chloromethyl-X-rosamine (MitoTracker Red) photosensitises mitochondria and induces apoptosis in intact human cells: T. Minamikawa, et al.; J. Cell. Science 112, 2419 (1999), Abstract;

Chloromethyltetramethylrosamine (Mitotracker Orange) induces the mitochondrial permeability transition and inhibits respiratory complex I. Implications for the mechanism of cytochrome c release: L. Scorrano, et al; J. Biol. Chem. 274, 24657 (1999), Abstract;