Total Nuclear-ID™ green/red nucleolar/nuclear detection kit for microscopy



ENZ-51006-500	1 Kit	220.00 USD

Simultaneous staining of both the nucleolus and the nucleus in live cells
High resistance to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods
Validated for utility in live cell imaging applications, demonstrating appropriate response to treatment with well characterized nucleolus-perturbation agents
Stringently manufactured, to control and eliminate non-specifi c assay artifacts

Enzo Life Sciences’ Total Nuclear-ID™ Green/Red Nucleolar/Nuclear Detection Kit contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei. The dyes allow examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.

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Product Specification

Quantity:	500 assays

Quality Control:	1. Absorption peaks: Nucleolar-ID™ Green Detection Reagent: λ_max= 439 ± 9 nm Nuclear-ID™ Red Detection Reagent: λ_max= 566 ± 4 nm 2. % purity by HPLC: Nucleolar-ID™ Green Detection Reagent: ≥ 93%; Nuclear-ID™ Red Detection Reagent: ≥ 93% 3. A sample from each lot of Total Nuclear-ID™ Green/ Red Nucleolus/Nucleus Detection Kit is used to stain HeLa cells using the procedures described in the user manual. Cells stained with Nucleolar-ID™ Green Detection Reagent show maximal fluorescence signal within the nucleoli, with only faint fluorescence throughout the nucleus and cytoplasm. Nuclear-ID™ Red Detection Reagent maximally stains the DNA in the cell nucleus. Cells induced by actinomycin D show reduction of nucleolar signal.

Kit/Set Contains:	Nucleolar-ID™ Green Detection Reagent, 50 µL Nuclear-ID™ Red Detection Reagent, 50 µL Actinomycin D Control, 125 µg 10X Assay Buffer, 15 mL

Application:	For visualizing nucleoli and nuclei simultaneously in living cells.

Long Term Storage:	-80°C

Use/Stability:	With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Handling:	Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	The Total Nuclear-ID™ Green/Red Nucleolar/Nuclear Detection Kit is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

General Literature References

Nucleolus: the fascinating nuclear body: V. Sirri, et al.; Histochem. Cell. Biol. 129, 13 (2008), Abstract;

Regulators of nucleolar functions: D. Hernandez-Verdun & P. Roussel; Prog. Cell Cycle Res. 5, 301 (2003), Abstract;

Immunocytochemical localization of nucleophosmin and RH-II/Gu protein in nucleoli of HeLa cells after treatment with actinomycin : K. Smetana, et al.; Acta Histochem. 103, 325 (2001), Abstract;