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LYSO-ID® Red detection kit (GFP-CERTIFIED®)

Acidic organelle-selective dye for live cell staining of  lysosomes
 
ENZ-51005-0100 100 tests 81.00 USD
 
ENZ-51005-500 500 tests 247.00 USD
Do you need bulk/larger quantities?
 
  • Novel acidic organelle-selective dye, suitable for live cell staining of lysosomes
  • Long wavelength red emission that is easily multiplexed with common fluorescent dyes (coumarin, FITC, Cyanine 3) and fluorescent proteins (BFP, CFP, GFP, YFP)
  • No photo conversion to a green emitting dye and no complicating metachromatic dual emission artifacts, facilitating analysis with GFP, FITC and other fluorescent probes
  • Highly resistant to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods
LYSO-ID® Red Lysosomal Detection Kit (GFP-CERTIFIED®) is an ideal choice for fluorescence co-localization imaging with GFP-tagged proteins, providing spectrally pure red signal. By contrast, many red-emitting lysosomal probes photoconvert to green fluorescent species or display metachromatic artifacts wherein emission occurs both in the red and green regions of the spectrum, leading to spurious results in GFP co-localization experiments [Freundt et al (2007) Cell Res. 17(11): 956- 958; Nadrigny et al (2007) Biophys J. 93(3):969-980]. The LYSO-ID® Red Lysosomal Detection Kit (GFP-CERTIFIED®)contains a proprietary acidic organelle-selective dye suitable for staining live cells. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. This kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). The LYSO-ID® Red dye has been validated for utility in live cell imaging applications, showing an appropriate response to treatment with well characterized lysosomal perturbation agents. A lysosome perturbation agent, chloroquine, is provided as a positive control for monitoring changes in lysosome number and volume. A nuclear counterstain (Hoechst 33342) is provided to highlight this organelle as well.

ENZ-51005 Fig1
Composite bright-field and fluorescence microscopy images of control U2OS cells (left) and cells pre-treated with 64μM Chloroquine for 5 hours (right). Cells were stained with LYSO-ID® Red dye for 10 minutes. Nuclei were counter-stained with Hoechst 33342 dye.

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ENZ-51005 Fig1

Product Specification

Applications:Fluorescence microscopy, Fluorescent detection
 
Application Notes:For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.
 
Quality Control:
  1. Absorption peak of LYSO-ID® Red Detection Reagent: λmax= 564 ± 9 nm
  2. % purity of LYSO-ID® Red Detection Reagent by HPLC: ≥ 93%
  3. A sample from each lot of GFP-CERTIFIED® LYSO-ID® Red Lysosomal Detection Kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells exhibit dramatic increase in lysosome-like vesicle number and volume in the chloroquine-treated HeLa cells when analyzed by microscopy.
 
Quantity:-500 size contains reagents for 500 assays
-0100 size contains reagents for 100 assays
 
Use/Stability:With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Kit/Set Contains:LYSO-ID® Red Detection Reagent
Hoechst 33342 Nuclear Staining
Chloroquine Control
10X Assay Buffer
 
Technical Info/Product Notes:The LYSO-ID® red lysosomal detection kit (GFP-CERTIFIED® is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.,

Cited samples: For an overview on cited samples please click here.
 

Product Literature References

Guanidinylated bioresponsive poly(amido amine)s designed for intranuclear gene delivery: J. Yu, et al.; Int. J. Nanomedicine 11, 4011 (2016), Abstract; Full Text
Induction of genomic instability and activation of autophagy in artificial human aneuploid cells: K. Ariyoshi, et al.; Mutat. Res. 790, 19 (2016), Application(s): Fluorescence microscopy using human normal breast epithelial MCF-10A cells, Abstract;
Novel guanidinylated bioresponsive poly(amidoamine)s designed for short hairpin RNA delivery: J. Yu, et al.; Int. J. Nanomedicine 11, 6651 (2016), Application(s): Flow cytometry using MCF7 cells, Abstract; Full Text
VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington’s disease: X. Guo, et al.; Nat. Commun. 7, 12646 (2016), Application(s): Immunocytochemistry to determine lysosomal activity, Abstract;
WO3/Pt nanoparticles promote light-induced lipid peroxidation and lysosomal instability within tumor cells: A.J. Clark, et al.; Nanotechnology 27, 075103 (2016), Abstract;
Analyzing the colocalization of MAP1LC3 and lysosomal markers in primary cells: K. Phadwal; Cold Spring Harb. Protoc. 2015, (2015), Abstract;
Autophagy limits proliferation and glycolytic metabolism in acute myeloid leukemia: A.S. Watson, et al.; Cell Death Discov. 1, Article number 15008 (2015), Abstract;
Clathrin-dependent endocytosis of claudin-2 by DFYSP peptide causes lysosomal damage in lung adenocarcinoma A549 cells: A. Ikari, et al.; Biochim. Biophys. Acta 1848, 2326 (2015), Abstract;
Identification and functional characterization of a solute carrier family 15, member 4 gene in Litopenaeus vannamei: Y. Chen, et al.; Dev. Comp. Immunol. 57, 57 (2015), Application(s): Stained drosophila Schneider 2 (S2) cells, Abstract;
Intracellular calcium levels as screening tool for nanoparticle toxicity: C. Meindl, et al.; J. Appl. Toxicol. 35, 1150 (2015), Application(s): Fluorescent Detection, Abstract; Full Text
Retinoic acid-induced IgG production in TLR-activated human primary B cells involves ULK1-mediated autophagy: A.B. Eriksen, et al.; Autophagy 11, 460 (2015), Application(s): Colocalization studies with anti-LC3B by confocal microscopy using human B cells, Abstract;
SerpinB2 (PAI-2) Modulates Proteostasis via Binding Misfolded Proteins and Promotion of Cytoprotective Inclusion Formation: J.A. Lee, et al.; PLoS One 10, e0130136 (2015), Application(s): Assay using murine embryonic fibroblasts, Abstract; Full Text
Threonine 56 phosphorylation of Bcl-2 is required for LRRK2 G2019S-induced mitochondrial depolarization and autophagy: Y.C. Su, et al.; Biochim. Biophys. Acta 1852, 12 (2015), Abstract;
Chromosome 19 open reading frame 80 is upregulated by thyroid hormone and modulates autophagy and lipid metabolism: Y.H. Tseng, et al.; Autophagy 10, 20 (2014), Abstract;
Macrophages eliminate circulating tumor cells after monoclonal antibody therapy: N. Gül, et al.; J. Clin. Invest. 124, 812 (2014), Abstract; Full Text
p38 signaling inhibits mTORC1-independent autophagy in senescent human CD8⁺ T cells: S.M. Henson, et al.; J. Clin. Invest. 124, 4004 (2014), Application(s): ImageStream analysis on PBMC, Abstract; Full Text
The c10orf10 gene product is a new link between oxidative stress and autophagy: M.W. Stepp, et al.; Biochim. Biophys. Acta 1843, 1076 (2014), Application(s): Lysosomal detection in African green monkey kidney cells (VERO cells), Abstract;
Epithelial uptake of flagella initiates proinflammatory signaling: D. Parker, et al.; PLoS One 8, e59932 (2013), Application(s): Detection by confocal microscopy, Abstract; Full Text
Inhibition of excessive mitochondrial fission reduced aberrant autophagy and neuronal damage caused by LRRK2 G2019S mutation: Y.C. Su, et al.; Hum. Mol. Genet. 22, 4545 (2013), Abstract;
Interactions between autophagic and endo-lysosomal markers in endothelial cells: C.L. Oeste, et al.; Histochem. Cell. Biol. 139, 659 (2013), Abstract;
A novel method for autophagy detection in primary cells: Impaired levels of macroautophagy in immunosenescent T cells: K. Phadwal, et al.; Autophagy 8, 677 (2012), Application(s): Lysosomal detection in PBMC using imaging flow cytometry, Abstract; Full Text
Cell death and autophagy under oxidative stress: roles of poly(ADP-Ribose) polymerases and Ca(2+): P. Wyrsch, et al.; Mol. Cell. Biol. 32, 3541 (2012), Application(s): Lysosomal detection in mouse embryonic fibroblasts using fluorescence microscopy, Abstract; Full Text
Role of ATG8 and autophagy in programmed nuclear degradation in Tetrahymena thermophila: M.L. Liu, et al.; Eukaryot. Cell. 11, 494 (2012), Application(s): Lysosomal detection in Tetrahymena using fluorescence microscopy, Abstract; Full Text
HspB8 Mutation Causing Hereditary Distal Motor Neuropathy Impairs Lysosomal Delivery of Autophagosomes: A.S. Kwok, et al.; J. Neurochem. 119, 1155 (2011), Abstract;
A Live-Cell Fluorescence Microplate Assay Suitable for Monitoring Vacuolation Arising from Drug or Agent Treatment: J. Coleman, et al.; J. Biomol. Screen. 15, 398 (2010), Abstract;

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