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Novel acidic organelle-selective dye, suitable for live cell staining of lysosomes
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Long wavelength red emission that is easily multiplexed with common fluorescent dyes (coumarin, FITC, Cyanine 3) and fluorescent proteins (BFP, CFP, GFP, YFP)
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No photo conversion to a green emitting dye and no complicating metachromatic dual emission artifacts, facilitating analysis with GFP, FITC and other fluorescent probes
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Highly resistant to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods
LYSO-ID® Red Lysosomal Detection Kit (GFP-CERTIFIED®) is an ideal choice for fluorescence co-localization imaging with GFP-tagged proteins, providing spectrally pure red signal. By contrast, many red-emitting lysosomal probes photoconvert to green fluorescent species or display metachromatic artifacts wherein emission occurs both in the red and green regions of the spectrum, leading to spurious results in GFP co-localization experiments [Freundt et al (2007) Cell Res. 17(11): 956- 958; Nadrigny et al (2007) Biophys J. 93(3):969-980]. The LYSO-ID® Red Lysosomal Detection Kit (GFP-CERTIFIED®)contains a proprietary acidic organelle-selective dye suitable for staining live cells. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. This kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). The LYSO-ID® Red dye has been validated for utility in live cell imaging applications, showing an appropriate response to treatment with well characterized lysosomal perturbation agents. A lysosome perturbation agent, chloroquine, is provided as a positive control for monitoring changes in lysosome number and volume. A nuclear counterstain (Hoechst 33342) is provided to highlight this organelle as well.
Composite bright-field and fluorescence microscopy images of control U2OS cells (left) and cells pre-treated with 64μM Chloroquine for 5 hours (right). Cells were stained with LYSO-ID® Red dye for 10 minutes. Nuclei were counter-stained with Hoechst 33342 dye.
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Product Details
| Applications: | Fluorescence microscopy, Fluorescent detection
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| Application Notes: | For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins. |
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| Quality Control: | A sample from each lot of LYSO-ID® Red detection kit (GFP-CERTIFIED®) is used to stain HeLa cells using the procedures described in the user manual. The chloroquine-treated HeLa cells exhibit dramatic increase in lysosome-like vesicle number and volume, comparing to untreated cells, when stained with LYSO-ID® Red Detection Reagent followed by microscopy analysis. |
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| Quantity: | -500 size contains reagents for 500 assays
-0100 size contains reagents for 100 assays |
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| Use/Stability: | With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | LYSO-ID® Red Detection Reagent
Hoechst 33342 Nuclear Staining
Chloroquine Control
10X Assay Buffer |
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| Technical Info/Product Notes: | The LYSO-ID® red lysosomal detection kit (GFP-CERTIFIED® is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.,
Cited samples: For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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