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Red hydrogen peroxide assay kit for microplates

 
ENZ-51004 1 Kit 273.00 USD
 
  • Flexible: Quantify hydrogen peroxide in solution, in cell extracts or directly from certain cells
  • Versatile: Detect a variety of oxidase activities through enzyme-coupled reactions
  • Sensitive: Detect as little as 10 picomoles of hydrogen peroxide
  • Complete: Mix and read reagents, with minimal hands-on time
  • Convenient: Homogenous assay format, fully compatible with HTS automation
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.
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Figure 1: Conversion of Red Peroxidase Substrate into Resorufin. Horseradish peroxidase uses Red Peroxidase substrate as an electron donor during the reduction of hydrogen peroxide to water. The resultant product, Resorufin, is a highly colored and fluorescent compound. (A) Use of assay for detection of glucose oxidase activity. (B) Use of assay in ELISA format to detect HRP-antibody conjugate.
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Product Specification

Applications:Fluorescence microscopy, HTS
 
Quality Control:A sample kit from each lot of Red Hydrogen Peroxide Assay Kit is used to detect H2O2 using the protocols described in the user  manual. Minimum Specifications: 1. A minimum of 10 picomoles of H2O2 must be detected in solution under normal conditions. 2. Fluorescence-based detection must yield H2O2 specific excitation and emission signals at 570 nm and 585 nm, respectively. 3. Absorbance-based detection must yield an H2O2 specific signal at OD576.
 
Quantity:

For 5 x 96-well plates.

 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Long Term Storage:-20°C
 
Kit/Set Contains:Red Peroxidase Substrate (1 vial)
Horseradish Peroxidase (HRP) (20 units)
Hydrogen Peroxide (H2O2) Stock Solution (200 µl)
DMSO (1 ml)
Assay Buffer (100 ml)
 
Background / Technical Information:This product is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 

Product Literature References

Middle-aged rats orally supplemented with gel-encapsulated catechin favorably increases blood cytosolic NADPH levels: M.E. Cueno, et al.; Phytomedicine (2015), Application(s): Assay,

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