Red hydrogen peroxide assay kit for microplates



ENZ-51004	1 Kit	247.00 USD

Flexible: Quantify hydrogen peroxide in solution, in cell extracts or directly from certain cells
Versatile: Detect a variety of oxidase activities through enzyme-coupled reactions
Sensitive: Detect as little as 10 picomoles of hydrogen peroxide
Complete: Mix and read reagents, with minimal hands-on time
Convenient: Homogenous assay format, fully compatible with HTS automation

Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.

Figure 1: Conversion of Red Peroxidase Substrate into Resorufin. Horseradish peroxidase uses Red Peroxidase substrate as an electron donor during the reduction of hydrogen peroxide to water. The resultant product, Resorufin, is a highly colored and fluorescent compound. (A) Use of assay for detection of glucose oxidase activity. (B) Use of assay in ELISA format to detect HRP-antibody conjugate.

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Product Specification

Quantity:	For 5 x 96-well plates.

Quality Control:	A sample kit from each lot of Red Hydrogen Peroxide Assay Kit is used to detect H₂O₂ using the protocols described in the user manual. Minimum Specifications: 1. A minimum of 10 picomoles of H₂O₂ must be detected in solution under normal conditions. 2. Fluorescence-based detection must yield H₂O₂ specific excitation and emission signals at 570 nm and 585 nm, respectively. 3. Absorbance-based detection must yield an H₂O₂ specific signal at OD₅₇₆.

Kit/Set Contains:	Red Peroxidase Substrate (1 vial) Horseradish Peroxidase (HRP) (20 units) Hydrogen Peroxide (H₂O₂) Stock Solution (200 µl) DMSO (1 ml) Assay Buffer (100 ml)

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Background / Technical Information:	This product is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.