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Red hydrogen peroxide assay kit

 
ENZ-51004 1 Kit 283.00 USD
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  • Flexible: Quantify hydrogen peroxide in solution, in cell extracts or directly from certain cells
  • Versatile: Detect a variety of oxidase activities through enzyme-coupled reactions
  • Sensitive: Detect as little as 10 picomoles of hydrogen peroxide
  • Complete: Mix and read reagents, with minimal hands-on time
  • Convenient: Homogenous assay format, fully compatible with HTS automation
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.
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Figure 1: Conversion of Red Peroxidase Substrate into Resorufin. Horseradish peroxidase uses Red Peroxidase substrate as an electron donor during the reduction of hydrogen peroxide to water. The resultant product, Resorufin, is a highly colored and fluorescent compound. (A) Use of assay for detection of glucose oxidase activity. (B) Use of assay in ELISA format to detect HRP-antibody conjugate.
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Product Specification

Applications:Fluorescence microscopy, HTS
 
Quality Control:A sample kit from each lot of Red Hydrogen Peroxide Assay Kit is used to detect H2O2 using the protocols described in the user  manual. Minimum Specifications: 1. A minimum of 10 picomoles of H2O2 must be detected in solution under normal conditions. 2. Fluorescence-based detection must yield H2O2 specific excitation and emission signals at 570 nm and 585 nm, respectively. 3. Absorbance-based detection must yield an H2O2 specific signal at OD576.
 
Quantity:

For 5 x 96-well plates.

 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:Red Peroxidase Substrate (1 vial)
Horseradish Peroxidase (HRP) (20 units)
Hydrogen Peroxide (H2O2) Stock Solution (200 µl)
DMSO (1 ml)
Assay Buffer (100 ml)
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 

Product Literature References

Effect of ZnO morphology on affecting bactericidal property of ultra high molecular weight polyethylene biocomposite: R.K. Sharma, et al.; Materials Science & Engineering C (2016), Application(s): Measurement of H2O2 released from the ZnO samples,
Localization of 14-3-3δ/ξ on the neuronal cell surface: Y. Yamamoto, et al.; Exp. Cell Res. 338, 149 (2015), Application(s): Measurment of Hydrogen Peroxide, Abstract;
Middle-aged rats orally supplemented with gel-encapsulated catechin favorably increases blood cytosolic NADPH levels: M.E. Cueno, et al.; Phytomedicine 22, 425 (2015), Application(s): Assay, Abstract;
Varying hemin concentrations affect Porphyromonas gingivalis strains differently: M. Ohya, et al. ; Microb. Pathog. 94, 54 (2015), Application(s): H2O2 measurements, Abstract;

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