Flexible: Quantify hydrogen peroxide in solution, in cell extracts or directly from certain cells
Versatile: Detect a variety of oxidase activities through enzyme-coupled reactions
Sensitive: Detect as little as 10 picomoles of hydrogen peroxide
Complete: Mix and read reagents, with minimal hands-on time
Convenient: Homogenous assay format, fully compatible with HTS automation
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.
Figure 1: Conversion of Red Peroxidase Substrate into Resorufin. Horseradish peroxidase uses Red Peroxidase substrate as an electron donor during the reduction of hydrogen peroxide to water. The resultant product, Resorufin, is a highly colored and fluorescent compound. (A) Use of assay for detection of glucose oxidase activity. (B) Use of assay in ELISA format to detect HRP-antibody conjugate.
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Product Details
Applications:
Fluorescence microscopy, HTS
Quality Control:
A sample kit from each lot of Red Hydrogen Peroxide Assay Kit is used to detect H2O2 using the protocols described in the user manual. Minimum Specifications: 1. A minimum of 10 picomoles of H2O2 must be detected in solution under normal conditions. 2. Fluorescence-based detection must yield H2O2 specific excitation and emission signals at 570 nm and 585 nm, respectively. 3. Absorbance-based detection must yield an H2O2 specific signal at OD576.
Quantity:
For 5 x 96-well plates.
Use/Stability:
Stable for at least one year after receipt when stored as recommended.
This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Evaluation of the Antioxidant Activity of Levetiracetam in a Temporal Lobe Epilepsy Model: I.I. Mejía, et al.; Biomedicines 11, 848 (2023), Abstract;
Mixtures of natural antimicrobials can reduce Campylobacter jejuni, Salmonella enterica and Clostridium perfringens infections and cellular inflammatory response in MDCK cells: I. Balta , et al.; Gut Pathog. 13, 37 (2021), Abstract;
Singlet oxygen generated by a new nonthermal atmospheric pressure air plasma device exerts a bactericidal effect on oral pathogens: Y. Hirano, et al.; J. Oral Sci. 61, 521 (2019), Abstract; Full Text
Effect of ZnO morphology on affecting bactericidal property of ultra high molecular weight polyethylene biocomposite: R.K. Sharma, et al.; Mater. Sci. Eng. C Biol. Appl. 62, 843 (2016), Application(s): Measurement of H2O2 released from the ZnO samples, Abstract;
Localization of 14-3-3δ/ξ on the neuronal cell surface: Y. Yamamoto, et al.; Exp. Cell Res. 338, 149 (2015), Application(s): Measurment of Hydrogen Peroxide, Abstract;
