ROS/RNS detection kit for microscopy



ENZ-51001-200	1 Kit	256.00 USD

Directly monitors reactive oxygen and/or nitrogen species (ROS/RNS) in live cells
Discriminates among superoxide, nitric oxide and peroxynitrite
High sensitivity, specificity and accuracy for live cell studies
Compatible with major components of tissue culture media (phenol red, FBS and BSA)
Complete set of reagents, including ROS/RNS inducers and scavengers

Enzo Life Sciences’ ROS/RNS Detection Kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. The kit includes three fluorescent dye reagents: NO Detection Reagent (red fluorescent), Oxidative Stress Detection Reagent (green fluorescent) for total ROS detection, and Superoxide Detection Reagent (orange fluorescent). It also includes the positive controls, pyocyanin and L-arginine, which are common inducers of ROS and NO production, respectively, as well as the negative controls, N-acetyl-L-cysteine and c-PTIO (common scavengers of ROS and NO, respectively).

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Product Specification

Quantity:	200 assays

Quality Control:	ROS/RNS Detection Kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard green (490/525 nm), orange (550/620 nm), and red (650/670 nm) fluorescent cubes. Stained HeLa cells induced with L-arginine exhibit bright red fluorescence with a red punctuate cytoplasmic staining pattern. L-arginine-induced cells incubated with NO Scavenger (c-PTIO) demonstrate minimal red fluorescence comparable to control values. Stained HeLa cells induced with pyocyanin exhibit bright orange signal in the nucleus as well as bright green fluorescence in the cytoplasm, but no red signal specific for nitric oxide. Pyocyanin-induced cells incubated with ROS Inhibitor (N-acetyl-L-cysteine) demonstrate minimal green or orange fluorescence signal comparable to control values.

Kit/Set Contains:	NO Detection Reagent (Red), 60µl Oxidative Stress Detection Reagent (Green), 250 nmoles Superoxide Detection Reagent (Orange), 250 nmoles NO Inducer (L-Arginine), 100 µl ROS Inducer (Pyocyanin), 100 µl NO Scavenger (c-PTIO), 400 nmoles ROS Inhibitor (N-acetyl-L-cysteine), 2x10 mg 10X Wash Buffer, 15 ml

Application:	This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy.

Long Term Storage:	-80°C

Use/Stability:	With proper storage, the kit components are stable up to the date noted on the product label. Store the kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Background / Technical Information:	The ROS/RNS Detection Kit is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

General Literature References

Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects: P. Wardman; Free Radic. Biol. Med. 43, 995 (2007), Abstract;

Fluorescence probes used for detection of reactive oxygen species: A. Gomes, et al.; J. Biochem. Biophys. Methods 65, 45 (2005), Abstract;

Determination of mitochondrial reactive oxygen species: methodological aspects: C. Batandier, et al.; J. Cell. Mol. Med. 6, 175 (2002), Abstract;

Methods of detection of vascular reactive species: nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite: M.M. Tarpey & I. Fridovich; Circ. Res. 89, 224 (2001), Abstract;