- Used for labeling of proteins and oligonucleotides
- Allows to immuno-tag molecules for detection using anti-digoxigenin antibodies with high affinities and specificity
Digoxigenin is a non-radioactive immune-tag, widely used for in situ hybridization and protein labeling. Pairing with anti-digoxigenin antibodies, digoxigenin tag allows the detection of interested biomolecules with high affinity and specificity. Digoxigenin NHS ester reacts with amino groups within biomolecules (nucleic acid, proteins, etc) and enables the attachment of digoxigenin into these molecules.
Product Details
Formula: | C35H50N2O10 |
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MW: | 658.78 |
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CAS: | 129273-26-3 |
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Purity: | ≥80% (HPLC) |
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Appearance: | Off-white solid. |
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Solubility: | Soluble in DMSO or DMF. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. |
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Handling: | Store in a dry place. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Detection and identification of human papillomavirus DNA in genital tract by digoxigenin labelling using the polymerase chain reaction method: S. Douvier, et al.; Clin. Diagn. Virol.
1(5-6), 325 (1994),
Abstract;
Detection of HBV-DNA using a digoxigenin-labelled probe. A rapid technique without loss of sensitivity: N. V. Naoumov, et al.; J. Hepatol.
12(3), 382 (1991),
Abstract;
Recent progress in the use of the technique of non-radioactive in situ hybridization histochemistry: new tools for molecular neurobiology: H. Kiyama, et al.; Neurosci Res.
9, 1 (1990),
Abstract;