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TRDMT1/DNMT2 monoclonal antibody (1E12)

 
ENZ-45019-0100 100 µg 416.00 USD
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Product Specification

Alternative Name:tRNA (cytosine(38)-C(5))-methyltransferase, PuMet
 
Clone:1E12
 
Host:Mouse
 
Isotype:IgG1λ
 
Immunogen:Recombinant full length TRDMT1 (DNMT2) (aa 1-392) fused to a GST tag.
 
UniProt ID:O14717
 
GenBank ID:AAH47733
 
Species reactivity:Human
 
Applications:ELISA, IF, WB
 
Recommended Dilutions/Conditions:ELISA (0.25-1µg/ml)
Immunofluorescence (5µg/ml)
Western Blot (0.5-1µg/ml). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application
 
Purity Detail:Protein A purified.
 
Formulation:Liquid. In 1x PBS, pH 7.2.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:In 2006, TRDMT1 enzyme was shown to methylate position 38 in aspartic acid transfer RNA and does not methylate DNA. To reflect this different function, the name for this methyltransferase was changed from DNMT2 to TRDMT1 (tRNA aspartic acid methyltransferase 1) to better reflect its biological function. TRDMT1 is the first RNA cytosine methyltransferase to be identified in a human.
 
ENZ-45019 WB
Figure: TRDMT1/DNMT2, mAb clone 1E12. Western Blot analysis of TRDMT1 expression in HeLa NE.
ENZ-45019 IF
Figure 2: Immunofluorescence of monoclonal antibody to TRDMT1 on HeLa cell. [Antibody concentration: 40µg/ml]
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ENZ-45019 WB ENZ-45019 IF

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