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DNMT Colorimetric Drug Discovery Kit

Rapid, sensitive, and simple colorimetric DNMT activity/inhibition kit.
 
ENZ-45017-0001 96 Reactions 587.00 USD
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  • Rapid - results in less than 4 hours
  • Simple and safe - Easy colorimetric assay without radioactivity or chromatography
  • Sensitive - measure as little as 0.5 μg of nuclear extract or 0.5 ng of purified enzymes
  • Specific - Optimized to allow high specificity to 5-mC without cross-reactivity to unmethylated cytosine
  • Reliable - reproducible results
DNMT Colorimetric Drug Discovery Kit is a simple method to detect DNA methylation activity or inhibition with results in <4 hours.
ENZ-45017 graph2
Figure 3. Demonstration of high sensitivity and specificity of DNMT activity/inhibition assay achieved by using recombinant DNMT1 with the DNMT Colorimetric Drug Discovery Kit.
ENZ-45017 schematic
Figure 1. Schematic procedure of the DNMT Colorimetric Drug Discovery Kit.
ENZ-45017 graph1
Figure 2. Demonstration of high sensitivity and specificity of the DNMT activity assay achieved by using nuclear extracts with the DNMT Colorimetric Drug Discovery Kit. Nuclear extracts were prepared from MCF-7 cells by using the EPIXTRACT® Nuclear Protein Isolation Kit (Prod. No ENZ-45016).
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ENZ-45017 graph2 ENZ-45017 schematic ENZ-45017 graph1

Product Specification

Alternative Name:DNMT Activity / Inhibition Assay
 
Assay Time:<4 hours
 
Applications:Colorimetric detection
 
Application Notes:For the quantitative determination of DNMT activity/inhibition in nuclear extracts or purified enzymes
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:Wash Buffer, DNMT Assay Buffer, Adomet, DNMT Enzyme Control, Capture Ab, Detection Ab, Enhancer Solution, Developer Solution, Stop Solution, 8-well Assay Strips with frame, Adhesive Covering Film,
 

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