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Universal methylated DNA standard (human)

Methylation standard (human) to help determine efficiency of a bisulfite DNA conversion
 
ENZ-45005-0001 20 tests 254.00 USD
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  • Conveniently use with methylation kits
  • Enzymatically modified standard includes short, easy to follow protocol
  • Primers included
The Universal human methylated DNA standard with the included primers can be used in conjunction with Enzo’s Blood and tissue methylation kit or the Express methylation kit. The DNA included in this set is enzymatically methylated at all cytosine positions to help assess the efficiency of the bisulfite-mediated conversion of DNA. DNA is isolated from a male human source. The primer set is designed to amplify a fragment of the human mismatch MLH1 mismatch repair gene following bisulfite treatment. The methylated cytosines comprising CG dinucleotides remain unconverted following bisulfite treatment, whereas non-methylated cytosines are converted into uracil and detected as thymine after PCR.
ENZ-45005 Fig2
The following primer set is provided in the pack. Following bisulfite treatment of experimental methylated DNA, PCR can be conducted using the hmLH1 set primers with the universal methylated DNA as a control.
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ENZ-45005 Fig2

Product Specification

Kit/Set Contains:Control primers: (hMLH1 (F) and hMLH1 (R)) 20µl each and Universal methylated DNA (human) 5µg
 
Formulation:Universal methylated DNA standard (human), 250ng/µl in TE buffer, pH 8.0.
Control primers 20µM each primer in TE buffer, pH 8.0
 
Use/Stability:With proper storage, good for one year upon receipt.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 

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